RE: [Histonet] Routine H & E stain,

2011-07-07 Thread amitapandey
Date: 07/07/11 07:10 PM Subject:RE: [Histonet] Routine H & E stain, I have always used Harris and have not experienced this problem? I do buy mine pre-made from VWR (cat#-95057-858). I agree with the xylene comment, also what mounting media are you using? The mounting media can

RE: [Histonet] Routine H & E stain,

2011-07-07 Thread Rittman, Barry R
@lists.utsouthwestern.edu; histonet; amitapan...@torrentpharma.com Subject: Re: [Histonet] Routine H & E stain, It seems that your mounting medium is acid. Try to correct that rather than changing the staining. René J. --- On Thu, 7/7/11, amitapan...@torrentpharma.com wrote: From: amitapan.

Re: [Histonet] Routine H & E stain,

2011-07-07 Thread Rene J Buesa
It seems that your mounting medium is acid. Try to correct that rather than changing the staining. René J. --- On Thu, 7/7/11, amitapan...@torrentpharma.com wrote: From: amitapan...@torrentpharma.com Subject: [Histonet] Routine H & E stain, To: "Histonet" ,

Re: [Histonet] Routine H & E stain,

2011-07-07 Thread kgrobert
Amita, We are a neurotoxicology lab that also provides histology services for other researchers inside & outside Rutgers, and we use Gill's #3 hematoxylin for our routine H&Es on mouse and rat tissues. No fading that I have noticed in all the years that I have been here. We get ours from Thermo

Re: [Histonet] Routine H & E stain,

2011-07-07 Thread SHANE NELSON
, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net From: "amitapan...@torrentpharma.com" To: Histonet ; histonet-boun...@lists.utsouthwestern.edu; histonet Sent: Wed, July 6, 2011 9:22:38 PM Subject: [Histonet] Routine H & E stain, Hello Hi

Re: [Histonet] Routine H & E stain,

2011-07-07 Thread SHANE NELSON
9:22:38 PM Subject: [Histonet] Routine H & E stain, Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide fo

[Histonet] Routine H & E stain,

2011-07-06 Thread amitapandey
Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result