Histonetters!
Our go-to stain is SafO for any bone/cartilage, but we always have the
option to use toluidine or alcian blue.
Just going out on a limb, but has anyone stained tissues with SafO/Fast
Green, without using any alcohols after the SafO step? These samples have
some issues when put into
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Safranin O cartilage staining
Hi,
We're having trouble staining murine articular cartilage with Safranin O. We
first started using this stain last year and it was working nicely, with strong
staining of both the articular cartilage and g
Hi,
We're having trouble staining murine articular cartilage with Safranin O. We
first started using this stain last year and it was working nicely, with strong
staining of both the articular cartilage and growth plate. After a while, the
stain stopped working, with staining only visible in the
Hi Liz,
Get it. Thank you for the detailed procedures.
Best Regards,
Victor
From: Elizabeth Chlipala
To: Victor Wong ; Tony Henwood (SCHN)
; "histonet@lists.utsouthwestern.edu"
Sent: Wednesday, February 20, 2013 12:15 AM
Subject: RE: [Histonet] Safranin O staining
Victor
wood (SCHN); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Safranin O staining
Hi Liz,
Thank you for your response and your invaluable experience in processing such
samples. I'll try manual processing on next batch of samples.
I am new to handle these samples. There would be a lot of c
Sent: Monday, February 18, 2013 11:37 PM
Subject: RE: [Histonet] Safranin O staining
We have processed and stained these types of samples before. We do not embed
the pellets in agarose prior to processing. We dye them with eosin and place
them in a tea bag and process on a very short cycle - 10 mi
r
sources.
P.S. unforuntuately we don't have a Haematology department but may be we can
try other cells.
Best Regards,
Victor
From: Tony Henwood (SCHN)
To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu"
Sent: Tuesday, February 19, 2013 6:44 AM
Subject: RE:
arose may be
deletrious to the staining. As suggested, I will extend the fixation time.
Best Regards,
Victor
From: "koelli...@comcast.net"
To: Victor Wong
Cc: histonet@lists.utsouthwestern.edu
Sent: Monday, February 18, 2013 10:42 PM
Subject: Re: [Histonet] Safranin O stain
ictor Wong ; "histonet@lists.utsouthwestern.edu"
Sent: Monday, February 18, 2013 8:42 PM
Subject: Re: [Histonet] Safranin O staining
Believe it or not, not because you are dealing with cells (small as they are)
they require longer than 1 hour to be correctly fixed.
This is what I wo
boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
(SCHN)
Sent: Monday, February 18, 2013 4:44 PM
To: 'Victor Wong'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safranin O staining
Hi Victor,
I suppose the take home message from Russ
ilto:vhlw...@yahoo.com]
Sent: Monday, 18 February 2013 6:36 PM
To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Safranin O staining
Dear Tony,
Thank you for your prompt reply and the paper.
I do fix the cell before processing in agarose and after embedding in a
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Wong
[vhlw...@yahoo.com]
Sent: Monday, February 18, 2013 12:35 AM
To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Safranin O staining
Dear Tony,
Thank you for your prompt reply and the paper.
I do fix
hen they
used.
Ray Koelling
Research Scientist
University of Washington
Seattle
- Original Message -
From: "Victor Wong"
To: histonet@lists.utsouthwestern.edu
Sent: Sunday, February 17, 2013 6:34:12 PM
Subject: [Histonet] Safranin O staining
Hi all,
I am workin
"histonet@lists.utsouthwestern.edu"
Sent: Sunday, February 17, 2013 9:34 PM
Subject: [Histonet] Safranin O staining
Hi all,
I am working on induced chondrogenesis in cell culture. After, induction, I
put the clump of cells (pellets) in 2% agarose to make a cell block. When the
pellet
by chondrogenic pellet can be destroyed by heat?
Best Regards,
Victor
From: Tony Henwood (SCHN)
To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu"
Sent: Monday, February 18, 2013 11:45 AM
Subject: RE: [Histonet] Safranin O staining
Victor,
Heat can affect the way ti
your processing
protocol?
Best Regards,
Victor
From: Tony Reilly
To: "histonet@lists.utsouthwestern.edu" ;
Victor Wong
Sent: Monday, February 18, 2013 1:50 PM
Subject: Re: [Histonet] Safranin O staining
Hi Victor
I have been using agar cell blocks for over 30 years. It h
Hi Victor
I have been using agar cell blocks for over 30 years. It has been my
experience that it is better to fix the cells in formalin prior to embedding in
the agar to prevent damage to the cells from the heat of the agar. Another
important step is to gently heat the agar so that it is ju
Hi all,
I am working on induced chondrogenesis in cell culture. After, induction, I
put the clump of cells (pellets) in 2% agarose to make a cell block. When the
pellet did not sink to the bottom of agarose, I heated to melt the agarose
again and centrifuged with higher speed. I fix the agar
Have you tried Sanderson's Rapid Bone Stain? Try using it first (7 minutes at
60C), then rinse in dH2O (a few dip an dunks at 60C and blot dry), then
counterstain with safranin O for 2-5 minutes (room temp - check intensity) and
rinse in 100% EtOH (room temp - few dip and dunks and blot dry). If
Hello everyone,
I am trying to stain for cartilage in mouse skull (cross-sectioning with
the brain in tact) and wanted to know if anyone might have a good
protocol for Safranin O / Fast green or Safranin O / von Kossa? The
tissue is embedded in MMA and was cut on a saw microtome and finished
down
Gotta second this. I've done a lot of PMMA and/or Spurrs-embedded
sectioning with it with good results.
Jack Ratliff wrote:
Yes, it works quite well for both demineralized and undemineralized
bone sections embedded in resin. I perform MMA embedding (not a kit)
and get really nice undemineraliz
Yes, it works quite well for both demineralized and undemineralized
bone sections embedded in resin. I perform MMA embedding (not a kit)
and get really nice undemineralized results. The resin I use allows me
the opportunity to deplastify the sections just as one would
deparaffinize when wor
Hello all.
Does Safranin O staining work on plastic sections (bone, decalcified)?
Any help would be appreciated.
Regards,
Markus
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