RE: [Histonet] Schiff mystery

2009-11-03 Thread Tony Henwood
AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, 2 November 2009 9:07 PM To: histonet@lists.utsouthwestern.edu Cc: 'Peggy Wenk' Subject: [Histonet] Sc

Re: [Histonet] Schiff mystery

2009-11-02 Thread Paula Pierce
tonet@lists.utsouthwestern.edu; Peggy Wenk Sent: Mon, November 2, 2009 2:55:43 PM Subject: Re: [Histonet] Schiff mystery There are several factors that influence the potency of Schiff's reagent. The quality of the basic fushsin. How much activated charcoal is used to decolorize the reagent. It is important

Re: [Histonet] Schiff mystery

2009-11-02 Thread Geoff McAuliffe
There are several factors that influence the potency of Schiff's reagent. The quality of the basic fushsin. How much activated charcoal is used to decolorize the reagent. It is important to use the minimum amount that gives a clear (or almost clear) solution. For the test, add a drop or two of

Re: [Histonet] Schiff mystery

2009-11-02 Thread Emily Sours
0010 > 202-476-4311 (office) > 202 476-4030 (fax) > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & > Peggy Wenk > Sent: Monday, November 02, 2009 05:07 > To: histonet@l

RE: [Histonet] Schiff mystery

2009-11-02 Thread Haynes, MaryAnne
histonet@lists.utsouthwestern.edu Cc: 'Peggy Wenk' Subject: [Histonet] Schiff mystery Need the help of the Histonet chemistry gurus. This has happened the last 2 times the students and I made up Schiff. Two different set of students. We make up the Schiff, and use it the next day, and it works correctly (sta

[Histonet] Schiff mystery

2009-11-02 Thread Lee & Peggy Wenk
Need the help of the Histonet chemistry gurus. This has happened the last 2 times the students and I made up Schiff. Two different set of students. We make up the Schiff, and use it the next day, and it works correctly (stains the glycogen in liver and neutral mucins in intestine a bright magenta