Hello,
I'm glad that you already solved the problem your way.
I didn't read your first post, but we had exactly the same problem (and
we're also using cover plates). This artifact is very likely caused by tiny
air bubbles which are trapped under the cover plate. The crucial step is
when you drip
n: do 25-2-2010 16:42
Aan: histonet@lists.utsouthwestern.edu; histonet.nos...@vneubert.com
Onderwerp: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides
To me it seems that the sections after being picked from the water bath were
not completely drained and the dewaxing proces
etely with water but the detergent will mix with the water
and will better remove the paraffin.René J.
--- On Thu, 2/25/10, histonet.nos...@vneubert.com
wrote:
From: histonet.nos...@vneubert.com
Subject:>[Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides
stonet.nos...@vneubert.com
To: histonet@lists.utsouthwestern.edu
Date: 02.04.2009 18:12:18
Subject: [Histonet] Strange circles in IHC slides
[...]> http://img12.imageshack.us/img12/8513/ts0402162049.jpg
> http://img13.imageshack.us/img13/6514/ts0402162104.jpg
[...]>
> So, has ever anyone experience
eppendorf repeat-pipettor (set at 2-mls)
for my washes (again, with buffer + detergent).
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
From: V. Neubert
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, April 2, 2009 12:12:18 PM
Subject: [Histonet] Strange circles
Hi
Looking at the images this appears to be the prozone effect which is caused by
the primary antibody concentration being too high and has been well reported in
both immunohistochemistry, immunology and serology articles. Do a google serch
for prozone phenomenon and you will find an explanat
It looks like bubble trapped between the slide and coverplate. Are you using
sufficient surfactant? That can help, but any of the "cap gap" methodologies
lend themselves to this problem.
Rinse your slides very thoroughly in buffer with plenty of surfactant before
loading them on the instrument
Those appear in all 14 slides I stained - I doubt to have made the same
mistake of leaving bubbles fourteen times when mounting them.
That my friend is the result of a air bubble on the section. If it had
been unremoved xylene the hematoxylin would have been absent as well.
I have seen some
boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of V.
Neubert
Sent: Thursday, April 02, 2009 11:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Strange circles in IHC slides
Hello,
I really have some real links to real pictures of real relevanc
Hi V.
That my friend is the result of a air bubble on the section. If it had
been unremoved xylene the hematoxylin would have been absent as well.
Cheers.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlotteto
Hello,
I really have some real links to real pictures of real relevance of the
real histonet list. Really promised.
http://img12.imageshack.us/img12/8513/ts0402162049.jpg
http://img13.imageshack.us/img13/6514/ts0402162104.jpg
So, has ever anyone experienced sth. like this?
My conjugate contro
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