Hey Charles, I am sure you have thought about this but your retrieval process could be too stringent if you are having over expression.
Thanks, Timothy Higgins, HT(ASCP), QIHC(ASCP)cm Histology Supervisor Center for Disease Detection/LabCorp tim.higg...@cddmedical.com Message: 6 Date: Wed, 19 Sep 2018 10:43:17 -0400 From: Charles Riley <cri...@dpspa.com> To: Histo List <histonet@lists.utsouthwestern.edu> Subject: [Histonet] braf (v6001e) Message-ID: <CAAQhB10DR0vi3Y=TLinNTpmUo4bpQ1F4mtjGd=vuaas+e-m...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Does anyone use this antibody on the Leica Bond systems? I have used several different vendors to validate this antibody but the Docs say all of them are over staining or not working correctly (I've gone down to 1:5000 on the dilution on all of them). If anyone has any suggestions on protocols or vendors that have worked for you (other than Ventana) please let me know. Thanks -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet