Carol,
I have heard of similar problems. I believe the temperature of your cryostat
may be one of the reasons for your problem along with the type of media.
Optimal cryostat temps for cutting unfixed tissue such as brain, lymph node,
liver and kidney should be -12- 16 degrees. Fatty tissue such a
Date: Wed, 16 Sep 2009 13:30:28 -0400
From: "Carol Buckingham"
Subject: [Histonet] TISSUE FREEZING MEDIUM
To:
Message-ID: <4ab0e874027e00013...@email.chop.edu>
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I'm having a great deal of trouble finding a tissue freezing medium t
I'm having a great deal of trouble finding a tissue freezing medium that is
anything like the original OCT -15-25. Since OCT is no longer available at
different temperature uses, I cannot cut a good section in our
cryostat(-25)without using freezing spray. Even after trying a few different
bran
