You can try to fill the TMA through the cassette from the bottom. Place the TMA in a mold that fits the TMA, heat it for about 2 min and then try filling the TMA from the cassette side. You can also dip the heated TMA block in a paraffin bath to fill the hole or use a plastic pipette to fill the gap. Anyway you get the paraffin to fill the hole you will need to melt the whole TMA for a bit to create a uniform block. If you have more questions give me a call.
Aprill Watanabe, B.S. Laboratory Coordinator Macromolecular Analyte Processing Center (MAPC) Integrated Cancer Genomics Division Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Office: 602-343-8822 Fax: 602-343-8840 Cell: 602-481-8654 email: awatan...@tgen.org website: www.tgen.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet