Sorry for the late response. I have done immunofluorescence on 50 um
cryosections of mouse host tumors and decalcified bone on super frost Plus
slides. I melt them to the slide using body heat then dry them at RT for a few
hours before storing at -80 deg C. They do not come off the slide. What
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Thursday, October 13, 2011 9:30 AM
To: 'Carmen Maria Garcia Pascual'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thick cryosections
I don't care what kink of slides you use if you cut 50 micron thi
Meter [mailto:montina.vanme...@pbrc.edu]
Sent: Thursday, October 13, 2011 9:46 AM
To: Patsy Ruegg; Carmen Maria Garcia Pascual;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thick cryosections
Carmen,
I routinely stain 40-60 micron sections for IHC. I currently run all my
IHC with a
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thick cryosections
I don't care what kink of slides you use if you cut 50 micron thick
sections and try to do IHC you are going to lose some or all sections,
in my experience. The only thing that might work and even this is
questionable because of the t
tonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carmen Maria
Garcia Pascual
Sent: Tuesday, October 11, 2011 9:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thick cryosections
Good afternoon everyone!
I have made 50um mice ov
Good afternoon everyone!
I have made 50um mice ovarian cryosections and I have used superfrost
plus slides. But some of them fall of the slides when I perfomed the
immuno, one colleague has told me that this is because the cuts are too
thick, and I think she is rigth. I don't know if there is
