[Histonet] Toluidine Blue Dilution

Hey Netters, Does anyone have a procedure for diluting Toluidine Blue from powder or 1% aqueaous with 10% NBF. I have always eyeballed the measurement as I was taught. However, as a new sup, I need to make sure we have an SOP in our manual. Thanks All, Natacha Mitchell, BS, HTL (ASCP) Histolo

[Histonet] Toluidine blue stain fo plant tissue

Could anyone give me the concentration (? 0.1-0.05%) and the exact technique/protocol to use. I am staining shoot apical meristems not leaf or root. Regards Peter Noyce PhD student. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://list

[Histonet] toluidine blue

Hi All- Is anyone using Toluidine Blue on air dried touch prep slides? Specifically for endobronchial ultra sound specimens (ebus). I am wondering if you can tell me what you are using for a toluidine blue recipe. Thank you for your help- Nancy Schmitt MLT, HT(ASCP) Pathology Support Services

Re: [Histonet] Toluidine blue stain for MMA

Yes. Probably hundreds of Histonetters stain plastic sections. Let us all hope they don't all bombard the Histonet listserver with replies to your question. Instructions for staining plastic sections with toluidine blue are in every library that contains books with paper pages, and also (albe

[Histonet] Toluidine blue stain for MMA

Hi, is there anyone have an experience with MMA toluidine staining? Im using T7200, T9100, Osteo-bed resin in lab now. Thanks, Kai Research Histotechnologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

Re: [Histonet] toluidine blue for cartilage with controls and mast cell staining

nfo/histonet> ] Sent: Tuesday, June 02, 2015 12:02 PM To: Histonet at lists.utsouthwestern.edu <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> Subject: [Histonet] Toluidine blue Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and w

Re: [Histonet] Toluidine blue

asked to review it. Lot of luck, Tony. From: Bernice Frederick [b-freder...@northwestern.edu] Sent: Wednesday, 3 June 2015 4:01 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello all, I was taught to do Toluidine Blue O without

Re: [Histonet] Toluidine blue

@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are foll

[Histonet] Toluidine blue

Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are following for this cartilaganous defect. Thanks, Bernice Bernice F

[Histonet] Toluidine blue background

We would like to use Toluidine Blue for staining frozen sections of kidney biopsy. We finally are able to get the stain dark enough, but we can't get rid of the background caused by the OCT.We've washed with water, alcohols, and PBS with no success. Is anyone out there using Toluidine Blu

[Histonet] Toluidine Blue stain for Mohs

Hi All, I am in need of a Toluidine Blue stain for Moh's Lab, would anyone be willing to share with me? It is for a friend. In advance, thank you very much. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com ___ Histonet m

RE: [Histonet] Toluidine blue

n.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, December 09, 2011 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Toluidine blue Anyone know Dutch? From: histonet-boun...@lists.utsouthwestern.edu on beha

RE: [Histonet] Toluidine blue

] Toluidine blue Anyone know Dutch? From: histonet-boun...@lists.utsouthwestern.edu on behalf of Bruijntjes, J.P. (Joost) Sent: Fri 12/9/2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Henk Al weer even geleden: weet je al iets

RE: [Histonet] Toluidine blue

Anyone know Dutch? From: histonet-boun...@lists.utsouthwestern.edu on behalf of Bruijntjes, J.P. (Joost) Sent: Fri 12/9/2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Henk Al weer even geleden: weet je al iets over de

[Histonet] Toluidine blue

Henk Al weer even geleden: weet je al iets over de toepassing van CD4 op varkensweefsel formaline gefixeerd en in paraffine ingebed weefsel? Heb nog een vraag: zijn jullie in het bezit van een cryostaat waar je nog met ouderwetse vaste messen kan snijden? Ik ben op zoek naar de mogelijkheid om

[Histonet] Toluidine Blue

Henk - Mast cells, on tissue and/or cytospins, are pH dependent - 2.0-2.5. Tissues are usually already fixed in formalin; cytospins can be fixed in 95% alcohol or formalin. I am sending our directions separately. The stain should less than 5 minutes totally. Cheryl _

[Histonet] Toluidine blue

Hi All, Have someone a good workong protocol for staining mastcells in cytospin slides with Toluidine Blue. Mention also the pH and fixation. Thanks, Henk van de Kant Utrecht University, Faculty of Science, Division of Pharmacology, David de Wied Building, Universiteitsweg 99, 3584 CG, Utrecht

[Histonet] Toluidine blue in decaled bone

We may need to do toluidine blue staining to assess cartilage changes on FFPE EDTA decalcified rat joints. We don't have experience w/ T blue and I have read the EDTA will extract proteoglycans. Help? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4

[Histonet] Toluidine blue staining of CFU-F

Hi all, I'm looking for a protocol to counterstain mouse CFU-F grown in tissue culture**. The protocols I've found just say they counterstained with this dye, don't say what it's dissolved in, sometimes list the percentage of it, and never list the time they stained for. The only full protocol I f

Re: [Histonet] Toluidine Blue and staining archaeological residues.

_ > From: Birgitta Stephenson > To: histonet@lists.utsouthwestern.edu > Sent: Mon, July 19, 2010 8:19:27 PM > Subject: [Histonet] Toluidine Blue and staining archaeological residues. > > Hello Histonetters, > > I am working with archaeological residues lifted

Re: [Histonet] Toluidine Blue and staining archaeological residues.

Starch (plants) + Iodine = Black solution. From: Birgitta Stephenson To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 8:19:27 PM Subject: [Histonet] Toluidine Blue and staining archaeological residues. Hello Histonetters, I am working with

[Histonet] Toluidine Blue and staining archaeological residues.

Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which

Re: [Histonet] Toluidine blue

Just be sure you get dye certified by the Biological Stain Commission. The bottle must have a Certification label or it is not certified to perform as expected. Sigma is reliable. Geoff zodia...@comcast.net wrote: Hello to all, I was wondering if anyone knows where to purchase toluidine bl

RE: [Histonet] Toluidine blue

I get mine from Sigma-Aldrich, it's pretty cheap for the dry chemical. Message: 12 Date: Wed, 21 Apr 2010 22:35:37 + (UTC) From: zodia...@comcast.net Subject: [Histonet] Toluidine blue To: histonet@lists.utsouthwestern.edu Message-ID: <1006938523.16905101271889337266.ja

RE: [Histonet] Toluidine blue

Newcomer Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of zodia...@comcast.net Sent: Wed 4/21/2010 5:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello to all, I was wondering if anyone knows where to

[Histonet] Toluidine blue

Hello to all, I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. Thank you in advance for your replies, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.uts

[Histonet] Toluidine Blue

I am currently writing a procedure for the use of Toluidine Blue staining for both FNA's and frozen sections at the request of one of our pathologists. Can anyone guide me in the use of this stain in lieu of Diff Quick or H&E for rapid processing (such as references I could use, etc) and does a

Re: [Histonet] Toluidine blue staining

To get consistent staining you must standardize your procedure. I make 1% aqueous Tol. Blue and 2% aqueous borax (sodium tetraborate) in separate containers. Each day I mix equal parts of each solution so I always use fresh stain. My final conc. of stain is 0.5%, doubling to 1% makes no differe

[Histonet] Toluidine blue staining

Hello. Our lab has had highly variable results using toluidine blue to stain 1.5 micron thick cross sections of plastic embedded nerve tissue from both mouse and human nerve biopsy. It seems like the stain is darker in the biopsy tissue than the mouse but both sometimes stain equally poorly. We ha

[Histonet] Toluidine Blue stain

Jen - We do T=blue staining almost daily. If you will contact me directly I will send you our directions. It is a very inexpensive stain, and can be reused. You will need to pH it often. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological