> The eosin will tend to leach out in the water rinses and the
differentiation solution
a touch of acid alcohol doesnt hurt either :)
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[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Haviland,Joie
Sent: Wednesday, 24 December 2008 7:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Understained my slides with hematoxylin
Hello:
I was doing H&E's and it was pointed out t
Hello:
I was doing H&E's and it was pointed out that I had understained the
hematoxylin. But the eosin was ok. How does one go back and restain the slides
and remove the old stain? Or do you use a differentiating solution?
Thanks for your help
Happy Holidays
Joie
_
