Not crazy at all, this is the kind of thing I was thinking someone would
have out there :)
I have used downey on hard tissue block faces before now too!
thanks so much for all your advice histonet :)
mills
On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet <
histonet@lists.ut
Hi Caroline,
This may sound a little crazy,
but I was working on some mummified tissue back in the 80's, (Mommy is old),
and to deal with the dried out tissue we would give them,
1. A quick .5 percent Sodium Hydroxide soak, 5minutes
2. Wash is running water, 1 minute
3. Soak in a 3 parts wate
Hi Millis,
Lets see if I’m understanding you correctly.
1) You have unprocessed tissue. What kind of unprocessed tissue & how thick are
the free-floating sections?
2) Are the tissue fixed? If so, with what? & For how long?
3) You want to stain the unknown whole mount using various histochemical
Hi there Histonetters!
I am currently working through some issues of unprocessing tissue to then
restain 'whole mount' with various stains, and then reprocess back to
paraffin.
I am running into issues of tissue being brittle and scratchy tissue after
reprocessing and sectioning.
Can anyone offe