Re: [Histonet] Unprocessing tissue

2016-09-26 Thread Caroline Miller via Histonet
Not crazy at all, this is the kind of thing I was thinking someone would have out there :) I have used downey on hard tissue block faces before now too! thanks so much for all your advice histonet :) mills On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet < histonet@lists.ut

[Histonet] Unprocessing tissue

2016-09-23 Thread Armstrong, Karoleigh T via Histonet
Hi Caroline, This may sound a little crazy, but I was working on some mummified tissue back in the 80's, (Mommy is old), and to deal with the dried out tissue we would give them, 1. A quick .5 percent Sodium Hydroxide soak, 5minutes 2. Wash is running water, 1 minute 3. Soak in a 3 parts wate

Re: [Histonet] Unprocessing tissue from paraffin

2016-09-21 Thread Maria Mejia via Histonet
Hi Millis, Lets see if I’m understanding you correctly. 1) You have unprocessed tissue. What kind of unprocessed tissue & how thick are the free-floating sections? 2) Are the tissue fixed? If so, with what? & For how long? 3) You want to stain the unknown whole mount using various histochemical

[Histonet] Unprocessing tissue from paraffin

2016-09-21 Thread Caroline Miller via Histonet
Hi there Histonetters! I am currently working through some issues of unprocessing tissue to then restain 'whole mount' with various stains, and then reprocess back to paraffin. I am running into issues of tissue being brittle and scratchy tissue after reprocessing and sectioning. Can anyone offe