Message: 3 Date: Mon, 15 Jun 2009 15:34:33 -0400 From: "Justin Peters" <jpet...@bostwicklaboratories.com> Subject: [Histonet] BCL-6 To: <histonet@lists.utsouthwestern.edu> Message-ID: <24d22de9e488aa43bf92a4389f2ddb1f05dfa...@mail1.bostwick.com> Content-Type: text/plain; charset="us-ascii"
I am having trouble getting bcl-6 antibody to work (Biocare CM223). Does anyone have a good protocol for me to try? Hi Justin, I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent. Most antibodies perform well with the "green" diluent; this particular antibody is best using the "RED". I also use Mach4 detection... if problems persist, contact me offline, I may be able to help with some other suggestions. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet