Dear Histonetter's,
Happy new year to everyone! I come today with a couple of questions regarding
human bone embedded in MMA:
1. What is a good technique to dissolve the MMA from the tissue section?
Also any suggestions on how to stain with H&E?
2. After mounting my tissue sect
it should never be
necessary to resort to a proprietary product whose composition is kept secret
from the buyer and user.
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
> From: Rene J Buesa
> Date: Monday, February 22, 2010 16:43
> Subject: Re: [Histonet
n, 2/22/10, Reuel Cornelia wrote:
From: Reuel Cornelia
Subject: [Histonet] Bone tissue
To: histonet@lists.utsouthwestern.edu
Date: Monday, February 22, 2010, 4:36 PM
We have a difficulty cutting metatarsal bone . It seems that our sections are
so dried up. I was thinking that our dehydr
We have a difficulty cutting metatarsal bone . It seems that our sections are
so dried up. I was thinking that our dehydration have something to do with this
which we have placed it in a wrong processing procedure for our large bone. The
tissue is 4 mm thick and 1-2 cm in length and width and w
500
> From: reuel.corne...@tsrh.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] bone tissue
>
> Can somebody help me with my bone tissue to remain intact during antigen
> retrieval for IHC staining. I have used subbed slides but It did not help me.
> Is there mo
Can somebody help me with my bone tissue to remain intact during antigen
retrieval for IHC staining. I have used subbed slides but It did not help me.
Is there more better adhesive. Does Haupts Gelatin works better? I know this
is a hundred year old question and hopefully this problem have been
