I always used Auramine at room temperature to identify TB bacilli in tissue
sections with fluorescence filter, never used heat and the results were as
expected. Bancroft is the one describing the procedure using heat for
auramine/rhodamine procedure but auramine alone at room temperature is
eno
I'm curious as to why we heat the Auramine Rhodamine when we are staining
tissue sections but our Micro lab stains at room temp. It is because "it's
always been done that way"?
Thanks group,
Angie
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