u] On Behalf Of DiCarlo,
Margaret
Sent: Tuesday, January 10, 2012 12:35
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] difficult cross section to cut
Histonetters,
I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
femur that they think is osteoporotic. After dec
@gmail.com]
Sent: Wednesday, January 11, 2012 1:37 AM
To: Histonet
Subject: Re: [Histonet] difficult cross section to cut
Hi Peggy
1. I have found that to cut bone the block has to be REALLY cold. Face
block with increased knife angle as suggested by Rene, and then place in
freezer overnight
Hi Peggy
1. I have found that to cut bone the block has to be REALLY cold. Face
block with increased knife angle as suggested by Rene, and then place in
freezer overnight - then try sectioning
2. Try surface decalcification of your block overnight in whichever soln.
you normally use.
3. Section at
Try increasing the knife cutting angle. The smaller the angle the more likely
the blade is going to skip over the cutting surface. If you are using around
10º, go to 30º angle.
René J.
--- On Tue, 1/10/12, DiCarlo, Margaret wrote:
From: DiCarlo, Margaret
Subject: [Histonet] difficult cross
Histonetters,
I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
femur that they think is osteoporotic. After decaling the cross section
of the femur in 10% formic acid for 12 days, processing using xylene and
embedding in Tissue Prep 2, I have been unsuccessful in attaining a
