Dear Histonetters, Is it technically possible to perform a double staining using alkaline phosphatase in both stainings ? I was thinking to perform a seqeuntial staining and develop the staining for the first protein and detect with a blue AP substrate and then perform staining for the second protein and detect with a red AP substrate. However, after the first staining, I would need to be able to completely block AP-activity or circumvent cross-reaction in some way.
Yves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
