What is the minimum time recommended for fixing routine endoscopy biopsies
before placing them on a short bx run program.
Thank you for your help
Fawn
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tonet] fixation question
Hello Histonetters:
Our lab has a resident wanting to start a project involving drug effect on
tumor size. Since he is wanting to compare tumor size at certain time
points after drug treatment (and do IHC staining intensity), he wonders how
much affect varying fixation times
Hello Histonetters:
Our lab has a resident wanting to start a project involving drug effect on tumor
size. Since he is wanting to compare tumor size at certain time points after
drug treatment (and do IHC staining intensity), he wonders how much affect
varying fixation times will have on the tiss
n.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret Blount
Sent: Monday, January 31, 2011 4:01 AM
To: Jan Berry; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] fixation question for IHC
It is possible to freeze paraformaldehyde in aliquots, so you don't have
riginal Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan
Berry
Sent: 28 January 2011 18:15
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fixation question for IHC
Is there a difference between using paraf
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From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Berry
Sent: Friday, January 28, 2011 1:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fixation question for IHC
Is there a difference between using paraformaldehyde
Is there a difference between using paraformaldehyde and neutral buffered
formalin when choosing a fixative for IHC? I would prefer to use formalin
because of easier preparation, but am willing to put in the extra time to make
fresh paraformaldehyde solution if there is a compelling reason.
Ja
Hi, i normally work on tissue.
I think 4% PFA in 0.1M PB is fine. so approach 3.
2009-04-08
TF
发件人： Guillermo Palchik
发送时间： 2009-04-08 05:03:13
收件人： histonet
抄送：
主题： [Histonet] Fixation question - Cerebellar granular cells
Dear Histoneters,
I am doing some IHC on rat cerebellar
Dear Histoneters,
I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and
I need to fix them with PF.
I have gotten 4 different answers on how to go about this, and I
wanted to run this by the list to see what you think.
1) Fix in COLD, 4% PF in 1x PBS
2) Fix in COLD, 4% PF i
