ebsite: www.tissueregenix.com<http://www.tissueregenix.com/>
Message: 5
Date: Tue, 24 Feb 2015 07:30:19 -0800
From: Yak-Nam Wang mailto:ynw...@u.washington.edu>>
Subject: Re: [Histonet] gelatin
To: John Kiernan mailto:jkier...@uwo.ca>>
Cc:
"histonet@lists.utsou
Dear ynw...@u.washington.edu.
Boiling makes steam, with bubbles that greatly change tissue structure. Slow
freezing is just as bad; the ice crystals make holes that deform and replace
the tissue architecture. What are you tryng to find out? It has been known for
100+ years that boiling collagen
Thank you for your e-mail.
Apologies for not explaining "treated tissue". We treat the tissue with
high intensity focused ultrasound. It can raise the temperature of tissue
to boiling in a localized area (millimeter areas). I could use a
biochemical assay for collagen and gelatin if we treat a lar
tonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan
Sent: Tuesday, February 24, 2015 12:48 AM
To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] gelatin
You need to explain "treated tissue".
Gelatin is co
You need to explain "treated tissue".
Gelatin is collagen that has been boiled until the protein has lost all its
fibrous nature and changed into a water-soluble protein. Gelatin is made
permanently insoluble by adequate formaldehyde fixation. It is stained by
anionic dyes (such as eosin in th
Hello,
Does anyone know of a stain specific for gelatin? I would like to
distinguish between firbous collagen and gelatin in treated tissue.
thank you
Yak-Nam
University of Washington
Seattle, WA
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Subject: [Histonet] gelatin beads
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 5, 2009, 10:19 AM
Good Morning,
I have an investigator who is bringing me rat brains that have been injected
with gelatin beads. I'm sectioning through the injection site(s) and she is
hopi
Good Morning,
I have an investigator who is bringing me rat brains that have been
injected with gelatin beads. I'm sectioning through the injection
site(s) and she is hoping to see the beads. BUT even though the
injection sites are easy to see there are no gelatin beads present.
My question is
