Thank you everyone for your help. It seems the consensus is to use acetic acid
as a mordant which worked. I have tried just blotting the tissue, but it never
seems to completely "dry". The tissue has been fixed in gluteraldehyde and
stained with the LacZ protocol and then post-fixed with PFA.
ists.utsouthwestern.edu
Subject: Re: [Histonet] histology ink washing off
Back in the old days we used to use "india ink" to label slides--so I don't
know whether it would also work on tissue (as opposed to glass), but it
might be worth a try. Susan
- Original Message -
#x27;Leslie Chen'" <[EMAIL PROTECTED]>
Cc:
Sent: Saturday, October 11, 2008 5:15 AM
Subject: AW: [Histonet] histology ink washing off
We dry and heat the tissue surface with a hair-dryer at the grossing. It
sounds a little bit rigid, but it works and the tissue margins are easily
see
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Leslie
Chen
Gesendet: Freitag, 10. Oktober 2008 22:31
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] histology ink washing off
We spray with a 3% solution of acetic acid.
Laurie
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Leslie
Chen
Sent: Friday, October 10, 2008 1:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] histology ink washing off
Hello,
I am having
- Fax
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Leslie
Chen
Sent: Friday, October 10, 2008 4:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] histology ink washing off
Hello,
I am having a problem with histology tissue marking ink wa
Hello,
I am having a problem with histology tissue marking ink washing off specimens
in water, PFA, or 70% alcohol. This is before the tissue even gets processed
for paraffin embedding. Does anyone have any suggestions on how to keep the ink
from coming off? Thank you.
Leslie
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