Re: [Histonet] hyaluronidase for IHC pretreatment

2009-09-16 Thread Geoff McAuliffe
You buy hyaluronidase (freeze-dried) from Sigma or some other vendor and make it up in the appropriate buffer. If my memory serves me, and there have been no major advances in the field, hyaluronidase comes in two flavors, one from bovine testis and one from Streptomyces sp. These are NOT the sa

[Histonet] hyaluronidase for IHC pretreatment

2009-09-16 Thread Amy Lee
Hello,   I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution. Could anybody help me please?   Best regards,   Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:/