I've made my own in situ buffer (for chromosome probes) using a simple
recipe:
5ml 2xSSC pH6.8
5ml formamide (deionized)
1g dextran sulfate (DS) Sigma D6001
Warm the 2xSSC and add the DS to dissolve. Add formamide. Sterile filter.
Aliquot and store at -20 C or lower.
Hope this helps!
Regard
Greetings, Oh mighty histonetters!
As per previous posts I am re-embarking on doing in situ hybridization. I
will be using (hopefully) DIG labelled oligo probes, and ultimately
visualizing with DAB.
My question isare there a commercially available hybrizidation buffers?
looking at the extensi
