Re: [Histonet] microtome issues: thick and no sections (Tyrone Genade)

2016-01-20 Thread Tyrone Genade via Histonet
Hello, Thanks to all who responded to my question. The solution, it seems was very simple. Several people reported similar experiences and issues with the specimen clamp not fitting tightly and that I should clean the clamp or block. My blocks are clean and I thought the clamp was too... but when

[Histonet] microtome issues: thick and no sections

2016-01-19 Thread Tyrone Genade via Histonet
Hello, I am having a problem with sectioning. I'm cutting 5 um sections but with the turn of the microtome I will miss the block, or take a small piece of the block/tissue and then the next section ends up thick. This missing can take several turns of the wheel so I can get really big sections. I