Fixation is not long enough and/or the piece of tissue is too large,
that is why you see problems at the center.
The connective tissue between the tubules is delicate and the normal
shrinkage due to paraffin processing may show some gaps
Fix longer, 24-48 hours is not sufficient.
A month in Boui
Thanks for the incoming info; it's a big help. Sorry I need a little bit
more detail so to explain a little more:
Some of the testis are showing gaps between the tubules, more towards
the centre of the testis and the researcher though it might be the
protocol but we saw the gap in subsequent tes
