(ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff
Sent: Tuesday, May 11, 2010 5:03 AM
To: Connolly, Brett M
Cc:
Subject: Re: [Histonet] need tips for cross-sectioning of cortical bone
Why not embed in resin (MMA) and take thicker sections and then grind/
polish them down? If you went this route, you could then use
flourescent labels and quantify mineral apposition rate and bone
formation rate. Let me know if you are interested. I can help you get
started and direct you to
out any wrinkles before transfering the sections to the
warm waterbath may also help.
I hope this helps!
Joe Saby, BA HT
From: Adam .
To: "Connolly, Brett M"
Cc: histonet@lists.utsouthwestern.edu
Sent: Thu, April 22, 2010 11:12:26 AM
Subject: Re: [H
the sections to the
warm waterbath may also help.
I hope this helps!
Joe Saby, BA HT
From: Adam .
To: "Connolly, Brett M"
Cc: histonet@lists.utsouthwestern.edu
Sent: Thu, April 22, 2010 11:12:26 AM
Subject: Re: [Histonet] need tips for cross-sec
Cutting bone is very hard, and I'm by no means an expert at it. Assuming the
blocks are properly fixed and decalcified, the best thing I've found is to
put the blocks at -20C for 5-10 mins to cool them, then right before you cut
them, rub a little ice water on the face of the block. That should hel
A colleague is having trouble getting wrinkle-free sections of
decalcified, paraffin embedded femur.
Any tips??
Thanks,
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com
