n.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Monday, April 28, 2014 5:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Negative controls
Hello fellow histonetters,
What is are other labs doing to satisfy the ANP.22570 QC - Antib
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Monday, April 28, 2014 4:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Negative controls
Hello fellow histonetters,
What is are other labs doing to satisfy the ANP
boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Monday, April 28, 2014 4:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Negative controls
Hello fellow histonetters,
What is are other labs doing to satisfy the ANP.22570 QC - Antibodies
"Appropriate negative controls
Hello fellow histonetters,
What is are other labs doing to satisfy the ANP.22570 QC - Antibodies
"Appropriate negative controls are used."
I apologize, I know this question has been asked before. I'm trying to
satisfy these requirements in one procedure.
Thank you all for your assistance!!
Bet
Hi All,
We are a Joint Commission inspected lab but after CAP changed their
requirements for negative controls we were hoping to drop them too. My manager
contacted TJC and asked what their position was regarding negative controls.
They responded that they have not changed their requirements
...@lists.utsouthwestern.edu] On Behalf Of
Abbott, Tanya
Sent: Tuesday, February 11, 2014 12:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Negative Controls for IHC
I just read in Advance Dec 2013 that there is a possibility of laboratories
utilizing fewer QC controls to cut costs? Has anyone
I just read in Advance Dec 2013 that there is a possibility of laboratories
utilizing fewer QC controls
to cut costs? Has anyone stopped running negative controls for IHC?
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph 610-
control for each
René J.
From: Ann Specian
To: histonet@lists.utsouthwestern.edu
Sent: Saturday, September 29, 2012 1:56 PM
Subject: [Histonet] Negative Controls in IHC
I have a question in regard to eliminating the use of negative controls when
using a non
bject: [Histonet] Negative Controls in IHC
I have a question in regard to eliminating the use of negative controls when
using a non-avidin-biotin detection system.
Do you not feel that negative controls may still need to be run in tissues
which are likely to be pigmented such as lymph node, skin and
I have a question in regard to eliminating the use of negative controls when
using a non-avidin-biotin detection system.
Do you not feel that negative controls may still need to be run in tissues
which are likely to be pigmented such as lymph node, skin and liver?
We were thinking to elimina
Does anyone know of any articles talking about not using negative
controls when using a polymer based detection system.
Judith Pardue
Memorial Hospital
Chattanooga, Tn.
judith_par...@memorial.org
This electronic mail and any attached documents are intended solely for the
named addressee(s)
rsday, August 02, 2012 8:35 AM
To: anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] negative controls
Yes you can, the cap standards just came out Tuesday with the revised
part stating you do not have to run a negative reagent control using
polymer based detection. Only if yo
rsday, August 02, 2012 8:35 AM
To: anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] negative controls
Yes you can, the cap standards just came out Tuesday with the revised
part stating you do not have to run a negative reagent control using
polymer based detection. Only if yo
@lists.utsouthwestern.edu
Subject: [Histonet] negative controls
just wondering if we can start leaving the negative controls off of our immuno
stains now as long
as our procedure says we can stain without them? we use ventanas multimer
detection systems.
thanks so much,
anita dudley
providence hospital
tonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita
dudley
Sent: Wednesday, August 01, 2012 4:13 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] negative controls
just wondering if we can start leaving the negative controls off of
just wondering if we can start leaving the negative controls off of our immuno
stains now as long
as our procedure says we can stain without them? we use ventanas multimer
detection systems.
thanks so much,
anita dudley
providence hospital
mobile, alabama
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher
Jacobs
Sent: Friday, July 13, 2012 9:31 PM
To: histonet@lists.utsouthwestern.edu
Cc: cjac...@clinpath.com
Subject: [Histonet] Negative controls and
Histonetters,
I have been made the IHC lead in a fairly large laboratory
that is seeking to become CAP accredited. I am definitely a newbie when it
comes to CAP. A question was brought up today about how we do our negative
controls. Specifically, should we run another negative control with any IHC
a monopoly. Let's
revolt. Seems to be catching on in the Middle East.
Joe
- Original Message -
From: "Angela Bitting"
To: "Diana McCaig" ; "Greg Dobbin" ;
; "Joyce Weems"
Sent: Wednesday, March 02, 2011 10:42 AM
Subject: RE: [Histonet]
e-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Greg
Dobbin
Sent: Wednesday, March 02, 2011 09:15
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] negative controls on immunos
Yes, it is always best to run ever
Behalf Of Greg
Dobbin
Sent: Wednesday, March 02, 2011 09:15
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] negative controls on immunos
Yes, it is always best to run every conceivable control available. Then
you can be really, really, really sure. However, if you have practical
issues that
@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin
Sent: Wednesday, March 02, 2011 09:15
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] negative controls on immunos
Yes, it is always best to run every conceivable control available. Then
Yes, it is always best to run every conceivable control available. Then you can
be really, really, really sure. However, if you have practical issues that come
into play such as cost of reagents, or extra controls taking up valuable space
on the stainer then you might have to think about what yo
If you do a run of several immunos today and you run a negative control,
and there is a request for additional immunos tomorrow, would you run
another negative control with the additional slides. They are being
stained on a stainer and not manually,
diana
_
nt: Friday, October 15, 2010 12:50 PM
To: Victoria Baker; Histo Net list server
Subject: RE: [Histonet] negative controls
Hi Vikki,
I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated to
each ultra. This means not all slides from any case are guaranteed to be on
the same mach
and any attachments immediately.)
- Original Message -
From: "Sebree Linda A"
To: ; "Rene J Buesa"
Cc: "Histo Net list server"
Sent: Friday, October 15, 2010 11:07 AM
Subject: RE: [Histonet] negative controls
To clarify even further: we cut 2 patient sec
...@lists.utsouthwestern.edu] On Behalf Of Victoria
Baker
Sent: Friday, October 15, 2010 10:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run
15, 2010 11:01 AM
To: sgoe...@xbiotech.com
Cc: Histo Net list server; histonet-boun...@lists.utsouthwestern.edu; Sebree
Linda A; Rene J Buesa
Subject: RE: [Histonet] negative controls
We're talking NEGATIVE controls here folks and if you use internal tissue
for your positive and negative cont
boratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com]
Sent: Friday, October 15, 2010 10:48 AM
To: Rene J Buesa
Cc: Histo Net list server; Sebree Linda A
Subject: RE: [Histonet] negative controls
So
Net list server
, Sebree Linda A
10/15/2010 11:47
AMSubject
RE: [Histonet] ne
ate them, and file them. So if you needed to see the HP
> control for October 15th, you could go pull the control for that day...
>
> Sarah Goebel, B.A., HT (ASCP)
> Histotechnician
>
>
> XBiotech USA Inc.
>
> 8201 East Riverside Dr. Bldg 4 Suite 100
>
> Austin, Tex
r that day...
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] negative controls
From: Rene J Buesa
Date: Fri, October 15, 2010 8:33 am
To: Sebr
test block?
The same goes for any bank of positives, so that is why you should have a
positive control section in the same slide as the test section.
René J.
--- On Fri, 10/15/10, sgoe...@xbiotech.com wrote:
From: sgoe...@xbiotech.com
Subject: RE: [Histonet] negative controls
To: "Sebree
)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
Date: Fri, October 15, 2010 8:08 am
To: "Victoria Baker" <[2]bakevict= o...@gmail.com>, "Histo Net list
server"
onet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run? Also, if you are doing this and have to use a different detection
kit
how do you work the QA/QC portion of this
Hi Vikki,
It's best to run it all together and run a negative control for each
detection kit.
Mark
On Fri, Oct 15, 2010 at 7:25 AM, Victoria Baker wrote:
> Hi
> I have a hypothetical question to those who run IHC on Ventana instruments.
> Are you running your negatives with your patient/test cas
se as long as it is on the same run.
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] negative controls
From: Victoria Baker
Date: Fri, Octob
@
lists.utsouthwest cc
ern.edu
Subject
[Histonet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with your patient/test cases or on a separate
run? Also, if you are doing this and have to use a different detection kit
how do you work the QA/QC portion of this for CAP requirements.
Th
] On Behalf Of Drew Sally A
Sent: 04 March 2010 16:02
To: Histonet
Subject: RE: [Histonet] Negative controls
Yes, Linda and I select the harshest protocol in the list of antibodies
we're running per case-we figure that the harshest will produce the
biggest problems with background, etc.
Sall
Thanks to all of you for your suggestions. We will be putting the Biocare
Universal Negative Control Serum through a trial.
Once again, Histonet proves it's not only invaluable, and populated by helpful,
knowledge and experienced histotechs, it's also extremely expedient!
Thanks,
Eric
Yes, Linda and I select the harshest protocol in the list of antibodies
we're running per case-we figure that the harshest will produce the
biggest problems with background, etc.
Sally Ann Drew, MT(ASCP)
IHC/ISH Clinical & Research Laboratory
UWHC
600 Highland Ave. DB1-223, Mail Code 3224
Madison,
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon,
Eric
Sent: Thursday, March 04, 2010 10:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Negative controls
Hi Anita,
We are currently examining the negative control issue in our laboratory.
We also
Hi Anita,
We are currently examining the negative control issue in our laboratory. We
also have Ventanas. We currently use a normal goat serum as our "non-immune"
serum. We are considering changing our SOP's to use the Ventana Neg Control
Rabbit and Neg Control Mouse in dispensers.
Linda
Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita
dudley
Sent: Wednesday, March 03, 2010 11:44 AM
To: histo...@pathology.swmed.edu
Subject: [Histonet]
we have a ventana benchmark and use rabbit and mouse negative controls, is
there anyone out there that uses a universal control and is that ok with your
cap inspection? thanks so much
antia dudley
providence hosp
mobile alabama
_
I don't see how negative controls for ordinary histochemical procedures would
be much help unless they are the same tissue as the test sample. If you are
doing a congo red for amyloid plaques in brain, I can see using a known
negative brain as a control. But I don't see the point of running a
I am reposting this because I didn't get any responses and am hoping for some
info from other JC-inspected labs
Tim
*
Hi all,
Our Joint Commission audit was just completed (first time for JC for me). We
passed almost everything fine.
The one thing they came up w
Hi all,
Our Joint Commission audit was just completed (first time for JC for me). We
passed almost everything fine.
The one thing they came up with is that we don't use "negative controls" for
most of the special stains - like trichrome, congo red, etc. We use negative
controls for micro-organ
Our Joint commission audit was just completed (first time for JC for me). We
passed almost everything fine.
The one thing they came up with is that we don't use "negative controls" for
most of the special stains - like trichrome, congo red, etc. We use negative
controls for micro-organism cases
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