RE: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Elizabeth Chlipala
-boun...@lists.utsouthwestern.edu] On Behalf Of Rachel M Gonzalez Sent: Monday, April 20, 2015 3:05 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] prevent wrinkles when cutting Hi The long ribbons are for R&D and QA purposes. Often we test under mult

Re: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Rachel M Gonzalez
; > From: j.benavi...@eae.csic.es > > To: histonet@lists.utsouthwestern.edu > > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > > > Hi there, > > > > I´m curious about the soaking thing. We have never done it in our lab. > > Which is the pur

Re: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Pam Marcum
.   Pam Marcum - Original Message - From: "Joelle Weaver" To: "Julio Benavides" , "Histonet" Sent: Monday, April 20, 2015 2:28:47 PM Subject: RE: [Histonet] prevent wrinkles when cutting Why are you cutting such a long ribbon? You usually only a

RE: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Joelle Weaver
stern.edu > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > Hi there, > > I´m curious about the soaking thing. We have never done it in our lab. > Which is the purpose to do it? > > Than, after facing the blocks, we chill them in a cold plate so, if > wanting t

RE: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Joelle Weaver
; To: histonet@lists.utsouthwestern.edu > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > Hi there, > > I´m curious about the soaking thing. We have never done it in our lab. > Which is the purpose to do it? > > Than, after facing the blocks, we chill them in a

Re: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread WILLIAM DESALVO
th tissue you can spare to practice your >> cutting techniques. I used to do this with my students and it really helped >> them. >> Good luck! >> >> Andi G. >> >> From: histonet-boun...@lists.utsouthwestern.edu >> [histonet-boun...@l

Re: FW: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Julio Benavides
on behalf of Rachel M Gonzalez [rac...@gbi-inc.com] Sent: Monday, April 20, 2015 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome I move to a lab that does not have someone dedicated to cut

RE: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Podawiltz, Thomas
Rachel M Gonzalez Sent: Monday, April 20, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no

FW: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Grantham, Andrea L - (algranth)
hwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of t

RE: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Marcum, Pamela A
@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of

RE: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Anne Murvosh
20, 2015 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections

[Histonet] prevent wrinkles when cutting

2015-04-20 Thread Rachel M Gonzalez
Hi Thursday was the first time I ever used a microtome I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of the original block. I am guessing they are wrinkling. What