> In what species was your primary antibody generated ?
>
>
>
> --- On Thu, 3/7/13, Nicole Cosenza wrote:
>
> From: Nicole Cosenza
> Subject: [Histonet] problem with DAB staining
> To: histonet@lists.utsouthwestern.edu
> Date: Thursday, March 7, 2013, 11:50 AM
>
>
Did you use a serum block ahead of your primary antibody ? If yes, what
species was it derived from?
In what species was your primary antibody generated ?
--- On Thu, 3/7/13, Nicole Cosenza wrote:
From: Nicole Cosenza
Subject: [Histonet] problem with DAB staining
To: histonet
Section should not dry in any stapes.
Tahseen
On 2013-03-07 22:50, Nicole Cosenza wrote:
Hello all:
I recently did IHC on paraffin embedded slides via the DAB method.
Upon addition of the DAB, the entire section turned brown. I know
this
is not true staining, as I don't expect a high yield
We would need to see the entire set of steps as there may be a variety of
causes.
Robert Schoonhoven, HT/HTL (ASCP)
From: Nicole Cosenza
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, March 7, 2013 12:50 PM
Subject: [Histonet] problem with DAB
Some suggestions:
1) your endogenous peroxidase was not quenched properly or entirely
2) your primary antibody is too concentrated
3) your secondary antibody is too concentrated
4) your incubation times are too long
5) your incubation temperatures are too high
6) your DAB was mixed up incorre
Hello all:
I recently did IHC on paraffin embedded slides via the DAB method. Upon
addition of the DAB, the entire section turned brown. I know this is not
true staining, as I don't expect a high yield of my protein of interest.
Any suggestions as to why this happened and what to do about it?
