] procedure for osmium and potassium chromate?
Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You
will need to look in an older methods book. The combo of dichromate and
osmium will not make your safety officer happy. There are many ways to
stain lipids with os
TREMELY dangerous and has to be used under extreme
precautions.
I don't think the results are worth the effort given the great dangers it
poses.
I for one would not share the procedure with you.
René J.
--- On Wed, 12/2/09, Liz Chlipala wrote:
From: Liz Chlipala
Subject: [Histonet] procedu
n Wed, 12/2/09, Liz Chlipala wrote:
From: Liz Chlipala
Subject: [Histonet] procedure for osmium and potassium chromate?
To: histo...@pathology.swmed.edu
Date: Wednesday, December 2, 2009, 3:13 PM
I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium
net-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, December 02, 2009 3:13 PM
To: histo...@pathology.swmed.edu
Subject: [Histonet] procedure for osmium and potassium chromate?
I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium dic
Ship to Address:
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Longmont, Colorado 80504
-Original Message-
From: Geoff McAuliffe [mailto:mcaul...@umdnj.edu]
Sent: Wednesday, December 02, 2009 1:41 PM
To: Liz Chlipala
Cc: histo...@pathology.swmed.edu
Subject: Re: [Histonet] procedure for osmium and potassium
Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You
will need to look in an older methods book. The combo of dichromate and
osmium will not make your safety officer happy. There are many ways to
stain lipids with osmium, alone or mixed with something else. Is this
for EM or LM?
I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium dichromate, does anyone out there have this
protocol available. I have found it before on the web but for some
reason I can't find it this time.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manag
