Hello everyone,
I’ve been fortunate enough to not deal with too many processor problems like a
processor breaking down mid schedule or someone putting the wrong chemicals in
wrong spot on the processor, but I want to plan for when these occurrences
inevitably happens. Does anyone have any resou
I have a back-up processor we keep filled for a back-up and if we have an extra
run. However with lower volumes lately we aren't using it. I was just wondering
about the paraffin in particularly. Does anyone know if you are not using it
how long it can stay there (its heated) before changing out
en Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, 19 April 2017 10:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] processor died overnight
Hello Histoworld,
I came in this morning to find that the processor died halfway through process
last nigh
, 19 Apr 2017 13:41:47 + (UTC)
From: Rene J Buesa
To: Lauren Sweeney ,
"histonet@lists.utsouthwestern.edu"
Subject: Re: [Histonet] processor died overnight
Message-ID: <174147213.3806623.1492609307...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
I
ssage-
From: Caroline Miller via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 19, 2017 12:17 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] processor died overnight
Yes, totally +1 to Rene, they should be fine.
(That has totally hap
Yes, totally +1 to Rene, they should be fine.
(That has totally happened to me too)!
Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet
> wrote:
>
> In 100% EthOL the tissues are completely "salvaged" and you can prepa
In 100% EthOL the tissues are completely "salvaged" and you can prepare the
program to continue the steps until melted paraffin.If there are delicate
tissue perhaps they will be "over-dried" but that is easily "compensated"
during microtomy.René
On Wednesday, April 19, 2017 9:01 AM, Lauren
Hello Histoworld,
I came in this morning to find that the processor died halfway through process
last night. The tissues are in 100% ETOH exactly half point. We do have a back-
up processor. In your professional experiences, would these tissues be
salvageable? Could I create a new program on th
Hi everyone,
For the past two months in the research lab I work in, we've been dealing with
poorly processed mouse livers. The following is the protocol, which is
optimized for mouse mammary tissue, but we've been using it for all tissues.
Fixation:
~48 hours in 10% nbf, shaking.
70% ethanol un
Hello Histonetters,
I was wondering if any of you have processor runs for 2 and 4 hour? I am trying
to run validations for our upcoming CAP inspection. The processor that we are
using is Tissue Tek VIP 5 Vacuum Infiltration Processor. This will greatly help
me out. Thanks in advance!
Diana
boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tina Van Meter
> Sent: Monday, December 01, 2014 10:15 PM
> To: Abbott, Tanya
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Processor
>
> Sakura VIP 5 or 6
>
> On
Agree with VIP for conventional tissue processing
Joelle Weaver MAOM, HTL (ASCP) QIHC
> Date: Mon, 1 Dec 2014 14:15:23 -0500
> From: tina.vanme...@gmail.com
> To: tanyaabb...@catholichealth.net
> Subject: Re: [Histonet] Processor
> CC: histonet@lists.uts
Sakura VIP 5 or 6
On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya <
tanyaabb...@catholichealth.net> wrote:
> Looking for a tissue processor to process 200-300 cassettes per run, any
> favourites?
>
> Tanya G. Abbott RT (CSMLS)
> Manager Technologist, Histology/Cytology
> St. Joseph Medical Center
Looking for a tissue processor to process 200-300 cassettes per run, any
favourites?
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph 610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net
This email and attachm
Hello Histoland,
We have an ASP300S processor that is not currently monitored by Leica. I
was wondering if anyone had an alarm system suggestion such that, in the
event it does malfunction, it will notify a designated person. We are a
private practice so there is no one in the building after "no
m (Denice Stiner)
7. VIP3000 reagent bottle (Mitchell Wan (Wanpost))
--
Message: 1
Date: Fri, 6 Jun 2014 17:52:27 +
From: Mike Tighe
Subject: [Histonet] Processor problem
To: "histonet@lists.utsouthwestern.edu
(
-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, June 06, 2014 1:56 PM
To: Mike Tighe; histonet@lists.utsouthwestern.edu
(histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Processor problem
My advise is to call Sakura and schedule a maintenance visit. The rotary
My advise is to call Sakura and schedule a maintenance visit. The rotary valve
may be "acting up".
René J.
On Friday, June 6, 2014 1:53 PM, Mike Tighe wrote:
I am having a problem with our tissue processor (Tissue Tek 3000). During
processing the first reagent (NBF) gets pumped into the
Yet another reason to go microwave. This can't happen.
- Original Message -
From: "Shirley A. Powell"
To: "Bill O'Donnell" , "Carla Conway"
, histonet@lists.utsouthwestern.edu
Sent: Friday, June 6, 2014 12:53:07 PM
Subject: RE: [Histo
t: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours
Not since 1978. (Sorry - couldn't resist - Happy Friday!)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Frida
I am having a problem with our tissue processor (Tissue Tek 3000). During
processing the first reagent (NBF) gets pumped into the retort without any
problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also
70%) however, gets about half way through pumping into retort and sto
bject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours
Hello everyone,
When it is working correctly, our tissue processor moves a basket of tissues
through 10 reagent containers. Last night it malfunctioned and suspended the
tissues above the 80% ethanol container. The tissues
rement day!
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Conway, Carla
[cmcon...@usgs.gov]
Sent: Friday, June 06, 2014 7:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction
Hello everyone,
When it is working correctly, our tissue processor moves a basket of
tissues through 10 reagent containers. Last night it malfunctioned and
suspended the tissues above the 80% ethanol container. The tissues were
high and dry for ~ 9 hours (!) until I placed them into 70% ethanol th
I posted the other day about having problems with my two processors. My
newest processor I believe is having an issue with
dehydration/infiltration. I have tried everything I know, plus some
suggestions from this list. I downloaded their manual for the processor and
the suggested program for this p
@lists.utsouthwestern.edu
Subject: [Histonet] Processor question - alcoholic formalin vs. buffered
formalin
I attended the Tri-State Symposium in Dubuque, IA last week (which was
fantastic) and sat in on a "Boot Camp for Histology" workshop. At this
workshop I realized that some problems we
I attended the Tri-State Symposium in Dubuque, IA last week (which was
fantastic) and sat in on a "Boot Camp for Histology" workshop. At this
workshop I realized that some problems we have been having with sectioning
could be due to our processor set up.
Before microtomy we have to soak our
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Hello all,
<<>>>
We use start at 95%, but we have two Pen Fix (alcohol fixative) steps between
our Formalin and alcohols. 10% NBF, 10% NBF, Pe
Hi Tom:
I deal exclusively with post-mortum brain tissue, so my situation may
not apply to you.
I do not use formalin on my processor, since the half brain used for
brain-cutting has already been thoroughly fixed.
So, I have the luxury of using 30%, 50%, 80%, 95%, then three 100%
Isopropanols.
;
Sent: Thursday, February 7, 2013 10:14 AM
Subject: [Histonet] Processor dehydration cycles..
Hello all,
I was wondering what most people use as the first reagent after the formalins
on their tissue processor? We have always used a sequence of 70%, 80%, 95%,
and 100% but is anyone using
Hello all,
I was wondering what most people use as the first reagent after the formalins
on their tissue processor? We have always used a sequence of 70%, 80%, 95%,
and 100% but is anyone using 80% or even 95% to start their dehydration?
Thanks in advance.
Tom McNemar, HT(ASCP)
Histology Co-o
Hi Sheila,
I used to install these for Leica. It's a very good machine and processes tx
nicely. I've put surgicals and bx's on the same run (8hr) during demo's and was
amazed at the results. I definitely recommend you do a demo.
Hope this helps.
Sent from the iPhone of Kim Tournear 🇺🇸
On Aug
Hello:We are looking for a new tissue processor. I've only ever used Sakura
products but the technology of the Leica ASP6025 looks like very good
technology.Has anyone used this processor? If so, can you give me your
opinion?Thanks :)Sheila
May I suggest?
Define a standard that works for you, combining both elements,
blocks and runs e.g.,
Every week or 200 blocks then check the first reagent volume and quality.
Again at 400
And again at 600 blocks.
We change the first reagent whenever it appears dirty.
Consider adding a few ccs. of
John,
Go for the ASP300S and then let's talk again at your convenience about resin
infiltration needs. I hope the information I sent to you was both informative
and helpful!
Regards,
Jack
On Mar 15, 2012, at 9:13 AM, John Baker wrote:
> Hello Jennifer, I saw your message about tissue pr
@lists.utsouthwestern.edu
Subject: [Histonet] Processor Preferences??
Hello Jennifer, I saw your message about tissue processors on the Histonet
Archive May 2006 and wondered which unit you decided to purchase? We are
looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s
and the
Of the 3 you mention, I would buy VIP6. Let's see what Jennifer answers.
René J.
--- On Thu, 3/15/12, John Baker wrote:
From: John Baker
Subject: [Histonet] Processor Preferences??
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 15, 2012, 10:13 AM
Hello Jennifer, I saw
Hello Jennifer, I saw your message about tissue processors on the Histonet
Archive May 2006 and wondered which unit you decided to purchase? We are
looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s
and the Tissue-Tek VIP6. Your thoughts on your choice and on these l
Design flaw in the screen display. It is in the way of the chamber when
opening chamber. If your not careful you will break the screen. Happens
fairly often.
Sent from my Windows Phone
From: Gauch, Vicki
Sent: 2/21/2012 9:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor
Hi everyone,
We are in the market for new processors...and I was wondering if anyone could
give me some pros and cons for the Tissue Tek VIP 6 tissue processor - how
reliable are they? Ease of use ? Any known problems? Tissues process well?
You knowall the usual questions we all ask for n
hwestern.edu
>> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
>> Sent: Tuesday, January 31, 2012 3:31 PM
>> To: histonet@lists.utsouthwestern.edu; Elizabeth Cameron
>> Subject: Re: [Histonet] Processor Recomendations?
>>
>> I used to work
gt; From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Tuesday, January 31, 2012 3:31 PM
> To: histonet@lists.utsouthwestern.edu; Elizabeth Cameron
> Subject: Re: [Histonet] Processor Recomendations?
&
: [Histonet] Processor Recomendations?
I used to work with 3 VIPs, one of which was 15 years old. I really doubt that
any of your VIPs "could go at any time".
I suggest that the money you have invest in refurbishing your VIPs, a capital
maintenance that the people from Sakura can do.
I
vesting in another instrument.
René J.
--- On Tue, 1/31/12, Elizabeth Cameron wrote:
From: Elizabeth Cameron
Subject: [Histonet] Processor Recomendations?
To: "histonet@lists.utsouthwestern.edu"
Date: Tuesday, January 31, 2012, 2:57 PM
Hi,
I am looking for a reasonably-priced ti
Hi,
I am looking for a reasonably-priced tissue processor for a research facility.
We only process mouse tissue (100-200/day), and rapid processing is not
something that is necessary. If possible, we would like the option of using
isopropyl alcohol for processing. We currently have 2 Sakura V
If anyone has a Shandon Excelsior ES and can offer me some help, I would
greatly appreciate it. I have one and have used it very little as it was the
back up processor. My old work horse, Pathcentre, is down and now the Excelsior
ES will be my main processor. I have zero training and zero instru
Does anyone in histoland have a list of types of specimens that a
histotechnologist who is processing specimens could be used for initial
competency.
Thanks,
Jimmy
Jimmy Lofton, M.S., HT,CT(ASCP)
Manager Histology Laboratory
Holy Cross Hospital
1500 Forest Glen Road
Silver Spring, MD 209
I am glad to hear the samples will be fine.
Thanks everyone!
Daniela Bodemer
Research Assistant
Surgical Research, Infection and Immunity
Murdoch Childrens Research Institute
The Royal Children's Hospital
Flemington Road Parkville Victoria 3052 Australia
T 03 9345 5930 T (03
Yes, nothing to worry about here (I am assuming an autotechnicon?). The first
paraffin would have dried and sealed the blocks keeping the tissue just fine,
however you are probably right in thinking they did not get enough paraffin
infiltration.
Try another hour in the second paraffin. If you
Hi all,
When I arrived the lab this morning I found the processor basket with my
cassettes stuck between the two paraffin tanks. I suppose it went
through the first cycle but not the second one, so its missing 1.5 hrs
of paraffin infusion. Should I put them back in hot paraffin for 1.5
hours or
Does anyone have any suggestions on power supply back-up for Leica ASP 300? We
are an independant lab and are not hooked up to back-up if we have an outage.
Thought it might be a good idea :)
Thanks.
___
Histonet mailing list
Histonet@lists.utsouthw
sgoe...@mirnarx.com
Sent: Monday, November 29, 2010 1:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor change
How many blocks do you guys normally process before you change the
solutions in the processor?
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
When the processor capacity is reached, I start to rotate the reagents,
discarding the first of each group, moving forward the remaining, and replacing
with fresh reagents the last.
René J.
--- On Mon, 11/29/10, sgoe...@mirnarx.com wrote:
From: sgoe...@mirnarx.com
Subject: [Histonet
, 2010 4:18 PM
To: sgoe...@mirnarx.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor change
I change solutions after 500 blocks; paraffin after 1500 blocks.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http
I change solutions after 500 blocks; paraffin after 1500 blocks.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
How many blocks do you guys normally process before you change the
solutions in the processor?
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
___
Hi
Hi All,
We are in need of a used tissue processor. If anyone has one for sale in the
Philadelphia area, please email ros...@email.chop.edu. Budget is very small!!!
Thank you.
Joanne Mauger HT(ASCP)QIHC
Children's Hospital of Philadelphia
From: histonet-bo
Happy Friday to all,
Our processor shut off last evening in 80% alcohol due to a misconnection of
the next reagent. We restarted this morning when we arrived but the tissues
were sitting in 80% for 9 hours. My question is what affect will this have on
the tissues which are a mixture of medulla
- Original Message -
From: "Kim Tournear"
To: "Histonet"
Sent: Tuesday, December 30, 2008 5:15 AM
Subject: Re: [Histonet] processor
Hi Jane,
We just bought 2 Peloris processors from Leica. We can process in as little
as 1 hour (usually biopsy specimens). Each mach
-Original Message-
From: Charles.Embrey
Sent: Monday, December 29, 2008 2:08 PM
To: 'kimtourn...@yahoo.com'
Subject: RE: [Histonet] processor
Pathos is made by Milestone not Leica.
Chuck
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
, AZ
~Don't let your life end before it begins~
OU Rocks
--- On Mon, 12/29/08, Jane C. Moose wrote:
From: Jane C. Moose
Subject: [Histonet] processor
To: histonet@lists.utsouthwestern.edu
Date: Monday, December 29, 2008, 7:10 AM
I know this question repeatedly occurs, but our t
I know this question repeatedly occurs, but our tissue processor "died"
last week. We are a small hospital lab doing about 2500 cases a year
(6000 specimens.) Would anyone have a recommendation on a new
processor for our size lab? An Excelsior was brought in for demo (not
sure what model) but
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