Re: [Histonet] query

2017-03-03 Thread Monson, Frederick via Histonet
uth Church St. West Chester, PA, 19383 fmon...@wcupa.edu 610-738-0437(Work) -Original Message- From: Nair, Indu via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, March 03, 2017 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] query Is there a p

[Histonet] query

2017-03-03 Thread Nair, Indu via Histonet
Is there a protocol to section un-decalcified bone? Or is there a laboratory that does this routinely? thank you indu - -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual

Re: [Histonet] Query on authorship

2016-08-20 Thread Rene J Buesa via Histonet
Jorge:The first thing is to be absolutely sure the data is worth publishing and that the results have scientific relevance.If this is the case and both you and the other contributor agree I think the data should be published.In no way you should eliminate the data obtained by the other individua

[Histonet] Query on authorship

2016-08-19 Thread Jorge A. Santiago-Blay via Histonet
Query on authorship Dear Colleagues: I am writing a small paper resulting from research done with two undergraduates many years ago (and, later on, involving several other colleagues using cutting-edge technology). As the results became obvious, both of the students agreed (orally, in person) wit

[Histonet] Query for Thionin/ Acid fucshin staining

2013-01-30 Thread Mohit Koladia
Hello histonetter, I was looking for the best protocol for thionin/acid fucshin staining. I was looking for details if someone has experience with same. I have paraffin embedded 5 micron sections of rat brains. Also, i want to know if thionin or cresyl violet makes some difference to the staining

[Histonet] Query Special Stainers

2010-10-06 Thread Joanne Clark
Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about

[Histonet] query regarding silde preparation for LCM

2009-04-12 Thread shazana hilda
Dear Histonetters, i've got few problem regarding the slide preparation for laser microdissection. for your information, i'm using a PEN-membrane from PALM to be used on laser microdissection pressure catapult (LMPC), Ziess. According to the protocol i need to UV the slide prior staining about 3

RE: [Histonet] query regarding IHC slide

2009-02-04 Thread Tony Henwood
histonet forum Subject: Re: [Histonet] query regarding IHC slide Dear Loralee, Unfortunately I'm using DAB and yet still faded. This is the first time I'm facing this problem. Thank You. Shazana Hilda ShamsuddinUniversiti Sains Malaysia Off. no: +609- 766 4440 Fax no: +609- 765

Re: [Histonet] query regarding IHC slide

2009-02-03 Thread shazana hilda
t forum Sent: Wednesday, February 4, 2009 11:37:17 AM Subject: Re: [Histonet] query regarding IHC slide What mounting media are you using? Are you dehydrating? How are you storing the slides? What DAB are you using (ie: source)? -Original Message- From: shazana hilda Date: Tue, 03 Feb 2

Re: [Histonet] query regarding IHC slide

2009-02-03 Thread anh2006
What mounting media are you using? Are you dehydrating? How are you storing the slides? What DAB are you using (ie: source)? -Original Message- From: shazana hilda Date: Tue, 03 Feb 2009 19:23:16 To: McMahon, Loralee A; histonet forum Subject: Re: [Histonet] query regarding IHC slide

Re: [Histonet] query regarding IHC slide

2009-02-03 Thread shazana hilda
_ From: "McMahon, Loralee A" To: shazana hilda Sent: Tuesday, February 3, 2009 11:47:06 PM Subject: RE: [Histonet] query regarding IHC slide [Histonet] query regarding IHC slide Are you using DAB or AEC. AEC fades and DAB does not. Loralee McMahon, HTL (ASCP) ICC Supervisor Uni

Re: [Histonet] query regarding IHC slide

2009-02-03 Thread shazana hilda
09 PM Subject: Re: [Histonet] query regarding IHC slide Please tell us what chromogen stains and counterstains you are using, and the type of mounting medium for the coverslip. This will help us try to answer your question. Jan Shivers UMN VDL - Original Message - From: "shazana

Re: [Histonet] query regarding IHC slide

2009-02-03 Thread Jan Shivers
09 8:10 PM Subject: [Histonet] query regarding IHC slide Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this ma

RE: [Histonet] query regarding IHC slide

2009-02-03 Thread Smith, Allen
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of shazana hilda Sent: Monday, February 02, 2009 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] query regarding IHC slide Dear All, Is anyone has experience with faded IHC-slide stained? I've d

[Histonet] query regarding IHC slide

2009-02-02 Thread shazana hilda
Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it? Any response are greatly appreciate