Hello to the Histotects community worldwide. Would like to know if someone out there has any suggestion or have worked with nematode larva:
1- fixation and after (protocol) 2-permeabilization of the chitin outer membrane (protocol) in order to: 3- adhere them to slides (maybe polylisine slides??? any other suggestion or protocol for slide adhesion of larva) 4- Run a immunohistochemistry on them (suggestions or protocol for any adjustments needed for further permiabilization and antigen retrieval if needed) Thanks Ana -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investigação de Recursos Naturais (CIRN) Secção de Anatomia e Taxonomia Zoológicas, Departamento de Biologia, Universidade dos Açores. Rua da Mãe de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (Açores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet