Re: [Histonet] rat kidney problems

2010-09-23 Thread Geoff McAuliffe
Greetings Abi: For cortical tubules (proximal convoluted tubules, PCT) to have a nice, open lumen one needs to fix by perfusion.This has been known for many years. Failing that, use a fix that works very rapidly in needed, alcoholic formalin for example, and slice the kidney into thin slices. W

[Histonet] rat kidney problems

2010-09-23 Thread abijag
Hello I need your advice regarding paraffin sections of rat kidney. Kidneys are collected, adequately fixed with 10 % NBF and undergone routine tissue processing in Sakura VIP 6 tissue processor.Sections are of 5 micron thickness. Strangely, in the H&E stained sections, our pathologists are com