Silly me: http://stainsfile.info/StainsFile/stain/hematoxylin/h-and-e-eo.htm
Bryan
Bryan Llewellyn wrote:
Read this page about eosin counterstaining on StainsFile.
Bryan Llewellyn
Cesar Francisco Romero wrote:
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here i
Read this page about eosin counterstaining on StainsFile.
Bryan Llewellyn
Cesar Francisco Romero wrote:
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here is the web page where you can find the formula.
http://protocolsonline.com/histology/dyes-and-stains/haematoxy
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here is the web page where you can find the formula.
http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/
I use it with very good results.
Hi Adrienne,
We see eosin leaching in our summer months when it is very humid in our lab.
The humidity is >70% for most of the summer. We change out all of our alcohols
daily to try and prevent it but we sometimes see it even with doing that.
Rhonda Gregoire, MLT
Supervisor, Clinical Patholog
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Eosin Leaching
I too have been experiencing Eosin leaching. I have tried all different
options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols
and xylenes that follow, even added a running water wash step after the Eosin (
to rem
I too have been experiencing Eosin leaching. I have tried all different
options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols
and xylenes that follow, even added a running water wash step after the Eosin (
to remove excess). Before this, I have had no problems and had ma
Ceri,
There are several ways to darken the eosin counterstain:
1. Stain longer in eosin, though with some eosins including eosin Y, this
might not necessarily darken the staining since the eosin Y is a yellow red,
eosin B seems to be more bluish red
2. Under-differentiate the haematoxy
Supposedly eosin used to color small specimens, or put in the
processor, fluoresces enough in tissue sections to interfere with
fluorescent stains.
I've seen safranin O (from the microbiology lab) used to mark small
surgical specimens like GI biopsies. I don't know whether you can put
it in the pr
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Sunday, September 09, 2012 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 106,
I just wanted to thank everyone who has responded to my message. All of the
input is greatly appreciated. This is a great site to be utilized in our quest
for Histology perfection.
Valerie
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington
We have used a mixture of Eosin Y and Phloxine B with acetic acid as follows:
780mL 95 %ETOH
100mL Eosin
10mL Phloxine B
4mL glacial acetic acid
We seem to be consistent. We have used this for 17 years.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
Wanda makes an excellent point, thinner sections have less contrast.
Geoff
On 8/9/2012 2:50 PM, wanda.sm...@hcahealthcare.com wrote:
How about the thickness of the tissue? Once we were cutting skins at 3 microns
and the pathologist complained the Eosin was too light so we started cutting
the
How about the thickness of the tissue? Once we were cutting skins at 3 microns
and the pathologist complained the Eosin was too light so we started cutting
them at 4-5 microns.
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Thursday, August 09, 2012 10:28 AM
To: Hannen, Valerie; histonet
Subject: [Histonet] RE: Eosin
Buy one with Phloxine in it. It will make it more red and
un...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Sarah Dysart
[sdys...@mirnarx.com]
Sent: Thursday, August 09, 2012 11:27 AM
To: Hannen, Valerie; histonet
Subject: [Histonet] RE: Eosin
Buy one with Phloxine in it. It will make it more red and stronger...
Sarah Goe
Buy one with Phloxine in it. It will make it more red and stronger...
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwe
Carol Freeman asks about using eosin and other dyes to mark small
specimens for better recovery during embedding.
I've found safranin O to be the best of these. Use the Gram stain
counterstain used in microbiology. You mark the specimens directly, on
those blue biopsy pads. It doesn't dissolve out
Here is a paper I found:
Capillary Electrophoresis Artifact Due to Eosin
Journal of Molecular Diagnostics, Vo. 7, No. 1, February 2005
Our molecular people are studying this issue to see how big of a problem it is.
Tim Morken
Supervisor, Electron Microscopy
Department of Pathology
UC San Franci
We also add liquid eosin (~75mL) to our "dirtiest" 100% to help aide the sight
of the small biopsies for embedding. You are correct though, that eosin does
fluoresce.
I understand why you would be concerned about FISH, so wanted to share this. I
have heard from fellow techs in the field that u
We use eosin in our last 100% alcohol on the processor. It is a great help for
our person embedding to be able to see and orient the small biopsies. We have
been doing this for years and have never had any problem with the processor or
any subsequent staining that has been performed. We also
We have not had any problem with fluorescence with our her 2 FISH or any
complaints from the Cytogenetics labs which run many other types of FISH. We
use eosin in our processors. The eosin will bleed out when you deparaffinize
the slides.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry S
>
>
>
> Robert Richmond
> Sent by: histonet-boun...@lists.utsouthwestern.edu
> 10/22/2010 10:44 AM
>
> To
> histonet@lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] Re: Eosin to dye small Biopsies
>
>
>
>
>
>
> Allison Scott at LBJ
hwestern.edu
cc
Subject
[Histonet] Re: Eosin to dye small Biopsies
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of
eosin to dye small biopsy specimens.
Several replies mention addition of eosin to one of the processing
alcohols. I have never seen this done, in maybe 60 pat
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of
eosin to dye small biopsy specimens.
Several replies mention addition of eosin to one of the processing
alcohols. I have never seen this done, in maybe 60 pathology services
I've worked in. (I'd know, because I nearly always exa
We put it in our 95%
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildrens.org
-Original Me
Hi Scott,
Your discription of eosin leaching out after a few days is classic for the
possibility of bleach contamination on your staining containers. There are
multiple possibilities of other causes, but this could be a simple cause,
easily solved.
Renee Grow, BA., HT (ASCP)
rg...@bmnet.com
His
Cheryl,
Yes, the Lecia XL is supposed to vibrate when it lifts the racks. If it's not
doing that, you need to call and ask for some assistance. It sounds to me like
there is something either turned off or broken in the setup.
Regarding the eosin getting the alcohol pink, that happens to us a
Thanks for all your help. It makes sense that it would evaporate faster on the
Leica as opposed to hand staining. The eosin on the Leica is uncovered for 6
plus hours a day when hand staining it was covered except when we were staining
a rack. I will add 100 alcohol as needed. Again thanks for a
: [Histonet] re; eosin
I have had some great suggestions from many of you, the problem is I am doing
literally all of them. My question is on the Leica XL isn't the instrument
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it
is keeping a lot of carryover becaus
I think that you should talk with the person who sold you this refurbished
instrument and present your problem. That will be more expeditious.
René J.
--- On Fri, 1/15/10, Cheri Miller wrote:
From: Cheri Miller
Subject: [Histonet] re; eosin
To: "histonet"
Date: Friday, January 1
I have had some great suggestions from many of you, the problem is I am doing
literally all of them. My question is on the Leica XL isn't the instrument
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why
it is keeping a lot of carryover because it isn't vibrating. Als
Cheri Miller
To: "Sherwood, Margaret" ; "Breeden, Sara"
; "Mahoney, Janice A" ;
histonet ;
"histonet-boun...@lists.utsouthwestern.edu"
Sent: Fri, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.
We do this as well, that is why I am
,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also tr
boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, January 15, 2010 10:22 AM
To: Mahoney,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
Eosin is alcohol-based,
Eosin is alcohol-based, thus the evaporation. The containers on my
autostainer get covered as soon as the last run is finished and that
slows evaporation somewhat but I do replenish as needed. I like my eosin
level in the container a little lower so we don't have pink-stained
frosted ends, but that
Hi Cheryl,
Maybe the auto stainer does not drain the rack a well or it could just be a
matter of the dipping and dunking that is causing more evaporation.
Jan
Omaha, NÉE
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
[lbla...@digestivespecialists.com]
Sent: Wednesday, July 01, 2009 1:47 PM
To: 'Jennifer Johnson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Eosin in alcohol
We use about the same amount or eosin in the first 100% alcohol and also do
nifer Johnson
Subject: [Histonet] Re: Eosin in alcohol
I have never heard of eosin interfering with IHC, as a matter of fact, I did
use it regularly and never had a problem with the IHC.
Perhaps there was another cause for the problems with the IHC.
rené J.
--- On Wed, 7/1/09, Jennifer Johnson
I have never heard of eosin interfering with IHC, as a matter of fact, I did
use it regularly and never had a problem with the IHC.
Perhaps there was another cause for the problems with the IHC.
rené J.
--- On Wed, 7/1/09, Jennifer Johnson wrote:
From: Jennifer Johnson
Subject: [Histonet] Re
I think they are probably getting weak unwanted staining with P504s/AMACR
antibody on benign glands on their PIN4s.
Since this antibody is usually pink/red, it sounds like someone might have
decided that the unwanted color came from eosin in the processor, but this
would be the wrong conclusion.
ly 01, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eosin in alcohol
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we recei
ieval process of staining
immunos.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson
Sent: Wednesday, July 01, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eos
Histologist, Southside Regional Medical
Center,
200 Medical Park Boulevard, Petersburg, Va. 23805, T: 804-765-5050, F:
804-765-5582, dkb...@chs.net
Jennifer Johnson
Sent by: histonet-boun...@lists.utsouthwestern.edu
07/01/2009 01:09 PM
To
cc
Subject
[Histonet] Re: Eosin in alcohol
We
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we received a letter from the lab that we send
our prostate biopsies to saying that it was undesirable bec
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