I find the same as Caroline Miller, about 20-25% with formalin fixation and
routine processing.
C
On Mon, Feb 22, 2016 at 3:41 PM, Caroline Miller via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> hi All, I have done some experiments in this area for mouse brains, and I
> find that ther
hi All, I have done some experiments in this area for mouse brains, and I
find that there is actually an expansion of tissue after formalin fixation
(around 10%), but then certainly a shrinkage to 100% dehydration agent of
about 20% from the original size. We found similar results with alcohol,
ace
LOL...Shrinkage...heh, heh.
But seriously, there should be little to no gross shrinkage from formalin
fixation and if the specimen is properly fixed, then there should be very
little gross shrinkage as it is dehydrated. That is supposed to be the point!
If someone is getting 30% shrinkage, the
...@comcast.net
Sent: Saturday, November 06, 2010 8:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] shrinkage during IHC
To All,
We are a lab that sends our specimens out for IHC and have just switched
to another reference laboratory for these services. Our
ject: SPAM-LOW: [Histonet] shrinkage during IHC
To All,
We are a lab that sends our specimens out for IHC and have just switched to
another reference laboratory for these services. Our pathologist is saying
that the tissue looks shrunk on the IHC slides, yet the slides th
To All,
We are a lab that sends our specimens out for IHC and have just switched to
another reference laboratory for these services. Our pathologist is saying that
the tissue looks shrunk on the IHC slides, yet the slides that I process (H&E,
and special stains)are fi
"'Edwards, Richard E.'" ,
cc
Subject
Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece
of string type question
Have you
Of Edwards,
Richard E.
Sent: Wednesday, August 25, 2010 7:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] shrinkage/a howlong is a piece of string type question
Many thanks to all who responded, for paraffin processed tissues the
figures suggested for the amount of shrinkag
Many thanks to all who responded, for paraffin processed tissues the
figures suggested for the amount of shrinkage found or expected were :- "more
than 5%":"5-10%":"10%"(twice):"10-15%":"20%":"25%":"30-35%":"30-40%", one
responder felt it was "noticeable" and another thought it was a
I seem to remember a good discussion in this in the following book:. L. P.
Kok and M. E. Boon. *Microwave Cookbook for Microscopists*, Coulomb Press
Leyden, Leiden (1992) p. 1–432 .
Whetehr or not it is still available is another matter
On Tue, Aug 24, 2010 at 5:17 PM, Edwards, Richard E.
wrote:
riginal Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, August 24, 2010 11:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] shrinkage
I think tissue shrinkage is a fair
- Original Message -
From: "Edwards, Richard E."
To:
Sent: Tuesday, August 24, 2010 11:17 AM
Subject: [Histonet] shrinkage
Anybody aware of the degree of shrinkage in paraffin processed tissues
and/or GMA processed tissues?, many thanks.
I was also taught 10% shrinkage in histo school.
Jay A. Lundgren M.S., HTL (ASCP)
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[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe
Sent: Tuesday, August 24, 2010 11:38 AM
To: Edwards, Richard E.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] shrinkage
Shrinkage for paraffin processed tissues is about 20%.
For GMA it is less than 5% which
endet: Dienstag, 24. August 2010 17:33
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] shrinkage
I have never seen a study, but when training, we were taught that
fixation and processing could result in up to 5-10% shrinkage.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology
Shrinkage for paraffin processed tissues is about 20%.
For GMA it is less than 5% which is one reason GMA stuff looks so nice.
Geoff
Edwards, Richard E. wrote:
Anybody aware of the degree of shrinkage in paraffin processed tissues and/or
GMA processed tissues?, many thanks.
I have never seen a study, but when training, we were taught that
fixation and processing could result in up to 5-10% shrinkage.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
bill.te...@pph.org
Voic
Anybody aware of the degree of shrinkage in paraffin processed tissues and/or
GMA processed tissues?, many thanks.
Cheers
Richard Edwards
Leicester University.
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