o: histonet@lists.utsouthwestern.edu
Subject: [Histonet] softening agent for tissues embedded in paraffin blocks
Dear List-members
I'm trying to cut some fish muscle samples (along with skin and some bone
tissues) embedded in paraffin (fixed in Bouin's fixative and decalcified with
EDTA)
] softening agent for tissues embedded in paraffin blocks
Your problem is not finding a "softener" but to decalcify your tissues
correctly. Usually histotechs rub decalcifying solutions over the exposed area
of the block but that wil affect the quality of the sections so, again,
decalcify pro
ds to make any
additional "softening".
René J.
From: Jose Luis Palazon Fernandez
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, September 26, 2012 5:56 AM
Subject: [Histonet] softening agent for tissues embedded in paraffin blocks
Dear List-me
Dear List-members
I'm trying to cut some fish muscle samples (along with skin and some bone
tissues) embedded in paraffin (fixed in Bouin's fixative and decalcified with
EDTA). I find it very difficult to cut these blocks and I would like to know,
based in your experience, what tissue softeni
