Anyone know a buffer or method (not using heat) to strip antibodies off cells
used in an immunocytostaining procedure?
The cells are in a multi-well tissue culture dish and have only been labeled
with primary (unconjugated) antibody.
Thanks.
Merced M Leiker
Cardiovascular Medicine
Biomedical Re
ubject: Re: [Histonet] Stripping antibodies
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sequential double staining.
Chris van der Loos
Academic Medical Center
Dept. of Pathology M2-230
Amsterdam
The Netherlands
Date: Sun, 30 Oct 2011 12:09:58 -0400
From: John Kiernan
Subject: Re: [Histonet] Stripping antibodies
To: histonet@lists.utsouthwestern.edu, cwes...@valasciences.com
Cc: A
; will try several of these techniques and evaluate which works the best.
>
> Thanks again for all your help,
>
> Claire
>
> --- jkier...@uwo.ca wrote:
>
> From: John Kiernan
> To: histonet@lists.utsouthwestern.edu, cwes...@valasciences.com
> Cc: Amos Brooks
>
Message: 2> Date: Thu, 27 Oct 2011 10:30:40 -0700> From: "Claire
Weston" <cwes...@valasciences.com>> Subject: [Histonet] Stripping
antibodies> To: <histonet@lists.utsouthwestern.edu>>
Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>> Content-Type:
e the colored end product. Then just start over
> for the new antigen.
>
> Good luck,
> Amos
>
> On Fri, Oct 28, 2011 at 11:50 AM, > wrote:
>
> > Message: 2
> > Date: Thu, 27 Oct 2011 10:30:40 -0700
> > From: "Claire Weston"
> > Subject:
p and dip the slide
in ethanol and it will remove the colored end product. Then just start over
for the new antigen.
Good luck,
Amos
On Fri, Oct 28, 2011 at 11:50 AM, wrote:
> Message: 2
> Date: Thu, 27 Oct 2011 10:30:40 -0700
> From: "Claire Weston"
> Subject: [Histonet
rea Marion
Graduate Student
University of Illinois at Chicago
[Histonet] Stripping antibodies
Claire Weston cweston <@t> valasciences.com
Thu Oct 27 12:30:40 CDT 2011
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Hi!
Does anyone have experience in IHC stripping antibodies from a tissue
section and re-probing with different antibodies? What is the best way to
do that? If you could share your stripping protocol or experience I would
appreciate it - thanks!
Claire
Claire Weston, PhD
Research
