Re: [Histonet] subbing positively charged slides

2015-07-09 Thread John Kiernan
Chrome-gelatin cannot be expected to stick well to a glass surface that has been modified by adding aminopropyl (or similar) groups to its silicate ions. See http://publish.uwo.ca/~jkiernan/adhesivs.htm. John Kiernan London, Canada = = = On 09/07/15, "Travers, Susan" wrote: > Does anyone know

Re: [Histonet] subbing positively charged slides

2015-07-09 Thread Geoff
Why would you want to do this? You could buy plain slides and sub them yourself. Geoff On 7/9/2015 8:45 AM, Travers, Susan wrote: Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just

[Histonet] subbing positively charged slides

2015-07-09 Thread Travers, Susan
Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just superstition... it seems as if the chrome alum might be positively charged itself and thus repelled from the surface of the slide...