Hi Gudren
I have not done this with TB but I have with other bacteria to produce controls
for gram stains. You need to have the tissue in a nutrient broth appropriate
for the organism. You need to consult with a laboratory that specialises in
Mycobacteria to find the appropriate medium to us
Hi all!
Can someone provide me with a procedure for selfmade Tb-control for
ZN-staining.
I tried incubating some lungtissue (2x2x2 cm) in a solution with 3-6 drops
of bacteria-suspension for 3 hours and for 18 hours. Afterwards fixing in
NBF for 4 days.
I had no success. No stainable mycobacter
