Re: [Histonet] tissue fixation-formaldehyde concentrations which is best.

2015-06-12 Thread Smith, Allen A
istonet] tissue fixation-formaldehyde concentrations which is best. Dear Peter, Some of the information you mention as "anecdotal" is wrong. Formaldehyde and paraformaldehyde are well documented in original peer-reviewed papers and in all textbooks in the fields of histotechnology and

Re: [Histonet] tissue fixation-formaldehyde concentrations which is best.

2015-06-06 Thread Tony Henwood (SCHN)
John, I totally agree Tony From: John Kiernan [jkier...@uwo.ca] Sent: Saturday, 6 June 2015 2:31 PM To: Peter Noyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations which is best. Dear

Re: [Histonet] tissue fixation-formaldehyde concentrations which is best.

2015-06-05 Thread John Kiernan
Dear Peter, Some of the information you mention as "anecdotal" is wrong. Formaldehyde and paraformaldehyde are well documented in original peer-reviewed papers and in all textbooks in the fields of histotechnology and histochemistry. Your anecdote about "high concentrations of formaldehyd

[Histonet] tissue fixation-formaldehyde concentrations which is best.

2015-06-05 Thread Peter Noyce
Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly known as 10% neutral buffered formalin)-in theory the 37% should fix quicker and better BUT anecdotally it is said that high concentrations of formaldehyde quickly form a "shell" in the tissue and will stop good penetration