Hi Betsy:
Wow, a real histology posting! If it is any comfort I have had the
same problem with mouse heart after Zenker's fix (I wanted nice
intercalated disks). I think the solution is to use NBF.
Geoff
Molinari, Betsy wrote:
Hi,
I processed some mouse hearts that were in Z-Fix for 24
Hi,
I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in
70% for anywhere from 24-48hrs. They were processed on a short run. The
epicardium looks good but the mid myocardium is wavy.
15 min 80%
15 min 95 x 2
15 min 100% x 2
15 min Xylenes x2
10 min paraffin x 4
Any sugg
