RE: [Histonet] weekend fixation

2010-05-21 Thread sgoebel
/span> (512)386-5107 Original Message Subject: RE: [Histonet] weekend fixation From: "Richard Cartun" <[1]rcar...@= harthosp.org> Date: Thu, May 20, 2010 8:07 pm To: <[2]linda.marg...@childr= ens.com>,<[3]mpe...@grhs.net&= gt;

RE: [Histonet] weekend fixation

2010-05-20 Thread Richard Cartun
lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Thursday, May 20, 2010 3:08 PM To: LINDA MARGRAF Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] weekend fixation >From what I have seen and heard you can have fixation times u

Re: [Histonet] weekend fixation

2010-05-20 Thread Mark Tarango
essage- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz > Chlipala > Sent: Thursday, May 20, 2010 2:54 PM > To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] weekend fixation > >

RE: [Histonet] weekend fixation

2010-05-20 Thread Mike Pence
inal Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, May 20, 2010 2:54 PM To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] weekend fixation I was talking to Peggy

RE: [Histonet] weekend fixation

2010-05-20 Thread Mike Pence
: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] weekend fixation From what I have seen and heard you can have fixation times up to=2 hours and still be ok? Sarah Goebel, B.A., HT (ASCP) <=iv> Histo=echnician XBiotech USA Inc. 8201 East Rivers=de Dr. Bldg 4

Re: [Histonet] weekend fixation

2010-05-20 Thread Mark Tarango
Sent: Thursday, May 20, 2010 1:04 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] weekend fixation > > > Histonetters: > Here's a message I was asked to post.. > > Dear Colleagues, > I have the following question concerning tissue processing. We d

Re: [Histonet] weekend fixation

2010-05-20 Thread Mark Tarango
ierlab.com >> >> >> Ship to Address: >> 1567 Skyway Drive, Unit E >> Longmont, Colorado 80504 >> >> -Original Message- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA >> M

Re: [Histonet] weekend fixation

2010-05-20 Thread Alexandra Meinl
We fix all specimens in NBF for 24h, wash them in tap water and store them in alcohol 70% at 4°C until further processing. That works perfectly well for us, and we never noticed any loss of staining. But I also knew that some colleagues prefer to run a weekend program on their Tissue Tek without de

RE: [Histonet] weekend fixation

2010-05-20 Thread sgoebel
6-5107 Original Message Subject: [Histonet] weekend fixation From: "LINDA MARGRAF" Date: Thu, May 20, 2010 12:04 pm To: Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing.

RE: [Histonet] weekend fixation

2010-05-20 Thread Liz Chlipala
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post.. Dear Colle

RE: [Histonet] weekend fixation

2010-05-20 Thread Liz Chlipala
] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot o

[Histonet] weekend fixation

2010-05-20 Thread LINDA MARGRAF
Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a pr