y RBC in the tissue).
Rene J Buesa wrote:
Antigen retrieval always comes first.
René J.
--- On Thu, 9/25/08, Monfils, Paul <[EMAIL PROTECTED]> wrote:
From: Monfils, Paul <[EMAIL PROTECTED]>
Subject: [Histonet] which one first - antigen retrieval or endo peroxidase
bl
Antigen retrieval always comes first.
René J.
--- On Thu, 9/25/08, Monfils, Paul <[EMAIL PROTECTED]> wrote:
From: Monfils, Paul <[EMAIL PROTECTED]>
Subject: [Histonet] which one first - antigen retrieval or endo peroxidase
block?
To: histonet@lists.utsouthwestern.edu
Date: Thursda
I'd say it doesn't matter which is 1st, except my inclination is always
to do the quench step 1st because the tissues seem a little less
delicate before retreival than after.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O.
When doing immunoperoxidase staining on a tissue that requires antigen
retrieval, which do you do first - the antigen retrieval or the endogenous
peroxidase blocking? Or does it matter?
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