Dear Abbey & Histonet I agree with René Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end.
Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ORIGINAL POST: Histonet Digest, Vol 89, Issue 21 Message: 7 Date: Tue, 19 Apr 2011 19:00:27 +1000 From: Abbey Mortimer <a.morti...@latrobe.edu.au> Subject: [Histonet] Tissue adhesion to slides Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.morti...@latrobe.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
