1, 2011 12:09 PM
> To: Galbraith, Joe
> Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ih...@googlegroups.com;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [IHCRG] RE: [Histonet] batching controls
>
> This is the most cautious approach, and for antibodies where there is no
yp...@mac.com
Sent: Tuesday, January 11, 2011 11:57 AM
To: Sebree Linda A
Cc: histonet@lists.utsouthwestern.edu; ih...@googlegroups.com; Taylor,Jean
Subject: Re: [IHCRG] RE: [Histonet] batching controls
I agree with Linda. One positive control per *batch*, not per case. The
positive control ti
Colbert; Taylor, Jean; ih...@googlegroups.com;
histonet@lists.utsouthwestern.edu
Subject: Re: [IHCRG] RE: [Histonet] batching controls
As you guessed correctly, I wasn't referring to analytical conditions. Control
tissues are often fixed at longer times than patient tissues. Secondly
; ih...@googlegroups.com;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [IHCRG] RE: [Histonet] batching controls
>
> This is the most cautious approach, and for antibodies where there is no
> internal controls that would be your only way to verify that the studies
> worked. But
Joe
-Original Message-
From: ancillaryp...@mac.com [mailto:ancillaryp...@mac.com]
Sent: Tuesday, January 11, 2011 12:09 PM
To: Galbraith, Joe
Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ih...@googlegroups.com;
histonet@lists.utsouthwestern.edu
Subject: Re: [IHCRG] RE: [Histonet] bat
estern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
ancillaryp...@mac.com
Sent: Tuesday, January 11, 2011 11:57 AM
To: Sebree Linda A
Cc: histonet@lists.utsouthwestern.edu; ih...@googlegroups.com; Taylor,Jean
Subject: Re: [IHCRG] RE: [Histonet] batching controls
I agree with Linda. On
This is the most cautious approach, and for antibodies where there is no
internal controls that would be your only way to verify that the studies
worked. But it's also associated with some unnecessary rejection of good
studies because controls aren't treated the same way as the patient slides.
I agree with Linda. One positive control per *batch*, not per case. The
positive control tissue can be placed on one of the patient's specimens, which
saves you money on the detection system and antibody as well.
We should be careful also about treating different drawers with different runs
(su
