Does anybody have a good protocol for decalcified teeth?
I will really appreciated
Luz
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Help! For years we have used Decal-Stat for decalcifying our bone marrow
biopsies with good results. For the past month we have been having problems
with tissue loss and morphological damage with these specimens following
decalcification. Unfortunately, this was just brought to my attention.
There was a paper
http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf
Talking about formic acid (Morse solution) can get as good result as EDTA in
ISH.
FYI.
Dorothy Hu
> Today's Topics:
>
> 1. Re. Decalcification with formic acid sodium citrate
> (Gayle Callis)
I have not found the need for neutralization.
With sufficient washing, common histochemical stains including H&E Gram
PAS-fungus and trichrome are spectacular.
Please explain your protocol for neutralization.
Steve A. McClain, MD
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Date: Sun, 19 Jul 2015 19:00:38 +
From: Steve McClain
To: ""
Subject: [Histonet] Decalcification
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One frequently overlooked point is washing after
One frequently overlooked point is washing after decal. Especially important
with acid decal, we wash for an hour in running water.
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this
communication.
-Original Message-
From: Swartwood, Steven J [mailto:steven.swartw...@cshs.org]
Sent: July-10-15 7:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Decalcification of mouse jaw and legs
Hello all,
I hope everyone is having or had a fantastic w
Hello all,
I hope everyone is having or had a fantastic week,
I'm going to test a few different decal methods on mouse jaws and leg bones. I
was wondering if anyone does this routinely out there who is willing to share a
protocol. I've never done this on mouse jaw/leg bones before. I've only do
Adrienne (where?) asks: "I have a really quick question: about how long
does it take to decal a bone marrow biopsy?" to which Jessica (where? -
apparently in the US though) replies "It all depends on what you use for
decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit
more time.
It is optional to decal in 4oC, but it is much better for preserve
antigenicity and get good result from enzymatic staining.
Similar way as you do fixation, you can choose RT or 4oC.
Dorothy Hu
Hello Histonetters
Is it imperative OR optional to maintain samples at below 4 degrees celcius
when us
Hello Histonetters
Is it imperative OR optional to maintain samples at below 4 degrees celcius
when using EDTA to decalcify bone samples
Thank You
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I have an adult bird skull that fixed with formalin and then has been stored in
70% ethanol.
I have seen the post that the sample stored in 70% ethanol can be walking back
through to series of ethanol to water and can be decalcified if it needs to be.
I am wondering if anybody has done this a
: [Histonet] decalcification of premolar teeth (dog)
Dear Members
I would be really interested to hear your advice on the currently preferred
procedure for decalcification of premolar teeth from dogs. Do laboratories
find the HCl/water solution or the formic acid solution or another solution
to be
Dear Members
I would be really interested to hear your advice on the currently preferred
procedure for decalcification of premolar teeth from dogs. Do laboratories
find the HCl/water solution or the formic acid solution or another solution
to be optimal for decal without obliterating the tissues to
hard bones and EDTA solution for bone marrow
biopsies.
I this you will better of in consulting a techniques book and follow the
instructions, especially those related to the "end point" of the
decalcification.
René J.
--- On Thu, 8/11/11, Rachael Glebocki wrote:
From: Rachael Glebocki
Hi Rachael,
I work with mouse bones on a regular basis, and I assure you that they are
incredibly difficult to work with, but with practice you can get decent
sections. It would be useful if you gave us a bit more information. What
kind of bones are you decalcifying? How are they fixed? How are yo
Dear Histonet users,
I was wondering if anyone has a operation procedure for bone decalcification
that works. I am having no joy in decalcifying the bone and making a good slide
from it.
Thank you for your time.
Rachael Glebocki
Teaching Technician
School of Veterinary Medicine & Science
Unive
: [Histonet] decalcification of articular cartilage
We do a lot of IHC staining on bone and cartilage samples and we use 10% formic
acid for decalcification for immunohistochemistry. I'm afraid you may not be
able to turnaround this in a week. The tissue needs to be adequately fixed
pri
-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz
Sent: Wednesday, April 20, 2011 8:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decalcification of articular cartilage
Dear Members,
We're planning to study sheep knee articular cartilage with histochemistry and
immunohistchemistry. Prox
Dear Members,
We're planning to study sheep knee articular cartilage with histochemistry and
immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde
at the moment. We need an effective, quick and cartilage tissue protective
decalcification method. We'll perform only light
:40:36
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Decalcification
We have small arteries that were FFPE and sectioned that should have been
decacified before processing. (The investigator did not indicate as such when
submitting them). Cutting was extremely difficult and the sections, as
Could you explain in more detail the process, Liz?
Thanks!
Peggy
-Original Message-
From: Liz Chlipala [mailto:l...@premierlab.com]
Sent: Wednesday, December 08, 2010 1:47 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Decalcification
Try
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Sherwood, Margaret
Sent: Wednesday, December 08, 2010 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Decalcification
We have small
We have small arteries that were FFPE and sectioned that should have been
decacified before processing. (The investigator did not indicate as such when
submitting them). Cutting was extremely difficult and the sections, as you can
imagine, had terrible knife marks and chatter. Of course, they ar
2010 10:14:35 AM
Subject: [Histonet] decalcification
I would appreciate any feedback on what all are using in your decalcification
process. We get a lot of large bones in and the past 2-3 months have noticed a
huge problem in our microtomy process with these samples. We have been
grossing
the
search Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124
412-268-8275 (o)
412-915-1683 (m)
412-268-9807 (fax)
smcbr...@andrew.cmu.edu
>
> --- On Mon, 8/2/10, Webb, Dorothy L wrote:
>
>
> From: Webb, Dorothy L
> Subject: [Histonet
You cannot set a fixed time for decalcification. You have to leave the bone in
the decalcifying solution until iy is soft and ready for processing.
René J.
--- On Mon, 8/2/10, Webb, Dorothy L wrote:
From: Webb, Dorothy L
Subject: [Histonet] decalcification
To: "
dg 4 = Suite 100
Austin, Texas 78744
(512)386-5107
Original Message ----
Subject: [Histonet] decalcification
From: "Webb, Dorothy L" <[1]dorothy.l.w...@healthpartners.com>
Date: Mon, August 02, 2010 7:14 am
To: "'[2]histo...@lists.u= tso
I would appreciate any feedback on what all are using in your decalcification
process. We get a lot of large bones in and the past 2-3 months have noticed a
huge problem in our microtomy process with these samples. We have been
grossing the bones in and leaving the sample in the cassette in 10
Dear all,
I am going to decal and process PIG femur and lumbar vertebrates. I only have
experience on soft tissues. Anyone can suggest any protocols in
decalcification and processing? Many thanks in advance.
Cheers,
Victor
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assistance or advice.
Best Regards,
Jack
> Date: Thu, 9 Oct 2008 15:17:07 +0200> From: [EMAIL PROTECTED]> To:
> histonet@lists.utsouthwestern.edu> Subject: [Histonet] Decalcification prior
> to Alizarine red or von Kossa possible?> > Hello all,> > I have to sta
Hello all,
I have to stain some specimens with bone formation in soft tissue. They want
me to do Alizarine red or Kossa stain.
But there are some heavily mineralized nodules in the tissue, I think they
can't be cut nicely without some kind of decalcification.
How should I treat these samples? Is t
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