Re: Re: Re: [Histonet] Counterstain for fluorescent tissue

2010-01-10 Thread John Kiernan
Neutral red (CI 50040) is an excellent fluorescent Nissl stain: 0.002%, in water, for 5 minutes; dehydrate, clear, and mount in DPX or another non-fluorescent resinous medium. With excitation by either near-UV or blue light (range 325-500nm) the Nissl substance and nuclei fluoresce yellow-orange

Re: Re: [Histonet] Counterstain for fluorescent tissue

2010-01-09 Thread TF
How about fluorescent nissl? 2010-01-10 TF 发件人: Nicholas David Evans 发送时间: 2010-01-10 02:16:57 收件人: Adam . 抄送: histonet 主题: Re: [Histonet] Counterstain for fluorescent tissue Dear Adam, Thanks very much for the very helpful advice. I guess my real question, which you're answered