What about section-thickness? Do you have a tech, who cuts very thin? I have sometimes this problem, that the staining looks very faint because the slide is just too thin.
Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von kristen arvidson Gesendet: Freitag, 12. Juli 2013 19:25 An: histonet Betreff: [Histonet] Eosin Staining Issues Hello All! I am having some bizzare staining issues. The eosin seems to be washing out?? The slides look "sun bleached" according to the Pathologists. The problem is that it is only happening on occasion. One rack stains fine and the next one has a problem. It even appears to be happening within cases staining in the same rack. My first though is tap water ph. We don't monitor that, haven't needed to, we've never had a problem like this before. Thoughts?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet