Sharon,
Your answer is probably in the following article in the Journal of
Histotechnology (Volume 32, number 2, June 2009):
"Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic
Activity and Morphology"
Authors: Iren Horkayne-Szakaly, Glenn D. Sandberg, Joren Keylock, El
We do everything you do, except we surround the tissue with oct in the tube
before freezing. Otherwise, it's freezer burn city. (in TX I think) :) We also
just write the info on the side of the bottle with a thin sharpie instead of
the cork thing. We usually store several tubes from the same ca
