Thanks all, looks like the consensus is that water into the clearing agent
causes it and I should change out alcohol more frequently.
Allan
On Wed, Oct 31, 2018 at 5:46 PM Allan Wang wrote:
> Hi histonet,
>
> For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%,
> 100%, 100
Richard W. Cartun, MS, PhD - Director, Histology & The Martin M. Berman, MD
Immunopathology & Morphologic Proteomics Laboratory - Director, Biospecimen
Collection Programs - Assistant Director, Anatomic Pathology, Hartford
Hospital, Hartford, CT asks
>>Anyone using ClearRite (xylene replacement) f
My answer is in the attached article I just published. Please read the
d-Limonene discussion seciton.
René J.
--- On Wed, 8/5/09, Sherwood, Margaret wrote:
From: Sherwood, Margaret
Subject: RE: [Histonet] Clear-Rite 3
To: "Va Paula Sicurello" , "Rene J Buesa"
, "H
ssage-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula
Sicurello
Sent: Wednesday, August 05, 2009 12:49 PM
To: Rene J Buesa; HistoNet
Subject: Re: [Histonet] Clear-Rite 3
Hi Rene,
Why do you not like the d-limonene solvents?
Sicurello
Sent: Wednesday, August 05, 2009 12:49 PM
To: Rene J Buesa; HistoNet
Subject: Re: [Histonet] Clear-Rite 3
Hi Rene,
Why do you not like the d-limonene solvents?
I work with them and the results seem fine. I have to mention that I use an
AutoTechnicon Duo and cannot place it in a fume hood to
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
C-MI for your imaging needs.
--- On Wed, 8/5/09, Rene J Buesa wrote:
From: Rene J Buesa
Subject: Re: [Histonet] Clear-Rite 3
To: Histonet
I use Formula 83 from CBG. I used Clear-Rite for quite a few years, but
Formula 83 works as close to Xylene as I have found.
Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941
-Original Message-
From: histonet-boun...@lists.utsouthwestern.
; BillO'Donnell
Subject: Re: [Histonet] Clear-Rite 3
Bill:
Xylene substitutes are not all "pretty much the same". There are d-Limonene
derivatives (something to stay absolutely away from) and alkane derivatives
with many known and unknown constituents.
Since you are about to change a
I would contact Anatech Ltd. they have a substitute "Pro-Par Clearant"
www.anatechltdusa.com - they are all very knowledgeable and would be able to
help you I am sure. I don't use substitutes in my lab at this time, however
I would contact them if I were ready to make the switch.
Amy S. Port
Bill:
Xylene substitutes are not all "pretty much the same". There are d-Limonene
derivatives (something to stay absolutely away from) and alkane derivatives
with many known and unknown constituents.
Since you are about to change and not have any preference, your best option
both in processing q
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