Kim: When you say 'complement C3' do you mean cleavage products of C3 after C3 activation (C3b-iC3b/C3c) or C3 iteself? C3b-iC3b/C3c are covalently attached to the surface of cells/pathogens, so I suspect this is what you would like to localize in your mouse tissue. We use an antibody in frozen sections that works beautifully (Cell Sciences clone 2/11). We have only limited experience with this clone in FFPE tissues. It likely requires antigen retrieval; thus far, we have tried using proteinase K and that was not successful. Because the frozen sections look so nice, we have not tried to localize C3b-iC3b/C3c in FFPE lately. If I had it to try again, I would start with citrate buffer antigen retrieval in my microwave pressure cooker.
Hope this helps. ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: prit...@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, January 14, 2009 1:23 PM To: Histonet Subject: [Histonet] Complement C3 for mouse FFPE Hi, Can anyone recommend a good antibody for complement C3 on mouse FFPE tissue? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet